Cheung Jimmy Ka-Wai, Bian Jingfang, Sze Ying-Hon, So Yee-Kiu, Chow Wing-Yee, Woo Chun, Wong Ms Tsz-King, Li King-Kit, Lam Thomas Chuen
Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, Hong Kong SAR, China.
Centre for Eye and Vision Research, Hong Kong SAR, China.
Data Brief. 2021 May 12;36:107120. doi: 10.1016/j.dib.2021.107120. eCollection 2021 Jun.
Water Gradient Contact Lens (WGCL) is a new generation material that combines the benefits of Silicone hydrogel (SiHy) and traditional hydrogel contact lenses by modifying the materials between the core and the surface. However, its impact on tear proteome has not been explored. Tears were collected on healthy young adults using Schirmer's strip at baseline, 1-week, and 1-month of WGCL lens wear (=15) and age-matched untouched controls (=10). Equal amounts of tears samples from individuals of WGCL and control groups were randomly pooled to form representative equal parts at each condition (=3 for WGCL wear and age-matched untouched control group) at each condition (baseline, 1-week, and 1-month). Tears were prepared using the S-Trap sample preparation followed by the analysis of a TripleTOF 6600 mass spectrometer. Using Information-dependent acquisition (IDA), a total of 725 tear proteins (6760 distinct peptides) were identified in the constructed spectral library at 1% FDR. Using data-independent acquisition (SWATH-MS), data were analyzed and processed using PeakView (v2.2, SCIEX), with the top differentially expressed proteins at each time point (baseline, 1-week, and 1-month) presented. All acquired raw data (IDA and SWATH-MS) were submitted and published on the Peptide Atlas public repository (http://www.peptideatlas.org/) for general release (Data ID PASS01589).
水梯度隐形眼镜（WGCL）是一种新一代材料，通过对材料的核心和表面进行改性，结合了硅水凝胶（SiHy）和传统水凝胶隐形眼镜的优点。然而，其对泪液蛋白质组的影响尚未得到探索。在健康年轻成年人佩戴WGCL隐形眼镜的基线、1周和1个月时（n = 15），以及年龄匹配的未佩戴对照组（n = 10），使用泪液试纸条收集泪液。将来自WGCL组和对照组个体的等量泪液样本在每个条件下（基线、1周和1个月）随机合并，以形成具有代表性的等量样本（WGCL佩戴组和年龄匹配的未佩戴对照组各3份）。使用S-Trap样本制备方法制备泪液，然后用TripleTOF 6600质谱仪进行分析。使用信息依赖采集（IDA），在1%错误发现率（FDR）的情况下，在构建的光谱库中总共鉴定出725种泪液蛋白质（6760个不同的肽段）。使用数据非依赖采集（SWATH-MS），数据用PeakView（v2.2，SCIEX）进行分析和处理，并呈现每个时间点（基线、1周和1个月）差异表达最显著的蛋白质。所有采集到的数据（IDA和SWATH-MS）已提交并发表在Peptide Atlas公共数据库（http://www.peptideatlas.org/）上以供公开发布（数据ID PASS01589）。
