Tsanaclis A M, Sesso A
Department of Pathology, School of Medicine, University of Sao Paulo, Brazil.
Pathol Res Pract. 1988 Sep;183(5):572-6. doi: 10.1016/S0344-0338(88)80013-X.
Somatotrophs from ten pituitary adenomas were evaluated morphometrically by light and electron microscopy using the following parameters: a) nuclear, cytoplasmic and cell volumes; b) volume density, total volume, surface density, total surface and surface/volume ratio of secretory granules, mitochondria, rough endoplasmic reticulum and Golgi apparatus, and c) the number of secretory granules and mitochondria per micron3 of cytoplasm and per cell. The results were compared (p less than 0.05 and p less than 0.10) with those obtained from somatotrophs identified in five normal pituitaries. The data obtained indicate that: a) in the adenomas, the number of secretory granules per cell cannot be accurately evaluated from their apparent number in sectioned cell profiles; b) there are two basic sub-types of adenomatous somatotrophs defined according to the mean secretory granule diameter; cells in which granule diameter is inferior to 180 nm exhibit distinct morphological features such as nuclear pleomorphism, the presence of gross bundles of intermediate sized filaments or fibrous bodies in the cytoplasm and a variable number of secretory granules. Adenomas constituted mainly by these cells were found in younger patients, suggesting the more aggressive nature of these tumours, thus warranting close clinical follow-up of such patients; and c) in both types of adenomatous cells, the organelles directly involved in the secretory process, i.e., the rough endoplasmic reticulum and Golgi apparatus, are larger than in the control cells; however, the ratio between the surfaces of these two compartments does not differ among the three groups studied.
利用以下参数，通过光学显微镜和电子显微镜对来自10例垂体腺瘤的生长激素细胞进行形态计量学评估：a）细胞核、细胞质和细胞体积；b）分泌颗粒、线粒体、粗面内质网和高尔基体的体积密度、总体积、表面密度、总表面积和表面积/体积比；c）每立方微米细胞质和每个细胞中的分泌颗粒和线粒体数量。将结果与从5例正常垂体中鉴定出的生长激素细胞所获得的结果进行比较（p<0.05和p<0.10）。所获得的数据表明：a）在腺瘤中，不能根据切片细胞轮廓中分泌颗粒的表观数量准确评估每个细胞中分泌颗粒的数量；b）根据平均分泌颗粒直径可将腺瘤性生长激素细胞分为两种基本亚型；颗粒直径小于180nm的细胞表现出明显的形态学特征，如核多形性、细胞质中存在粗大的中等大小细丝束或纤维体以及数量可变的分泌颗粒。主要由这些细胞构成的腺瘤多见于年轻患者，提示这些肿瘤具有更强的侵袭性，因此对此类患者需要密切的临床随访；c）在两种类型 的腺瘤细胞中, 直接参与分泌过程的细胞器，即粗面内质网和高尔基体，比对照细胞中的更大；然而，在研究的三组中，这两个区室的表面积之比没有差异。
