Research Team "Viruses, Hepatology, Cancer," Institut Mondor de Recherche Biomédicale, INSERM U955, Créteil, France.
Department of Virology, Hôpital Henri Mondor, Université Paris-Est, Créteil, France.
Antimicrob Agents Chemother. 2020 Jun 23;64(7). doi: 10.1128/AAC.00876-20.
Cyclophilins play a key role in the life cycle of coronaviruses. Alisporivir (Debio 025) is a nonimmunosuppressive analogue of cyclosporine with potent cyclophilin inhibition properties. Alisporivir reduced SARS-CoV-2 RNA production in a dose-dependent manner in Vero E6 cells, with a 50% effective concentration (EC) of 0.46 ± 0.04 μM. Alisporivir inhibited a postentry step of the SARS-CoV-2 life cycle. These results justify rapidly conducting a proof-of-concept phase 2 trial with alisporivir in patients with SARS-CoV-2 infection.
亲环素在冠状病毒的生命周期中发挥着关键作用。阿利司匹韦（Debio 025）是一种非免疫抑制性环孢素类似物，具有很强的亲环素抑制特性。阿利司匹韦以剂量依赖的方式降低了 Vero E6 细胞中的 SARS-CoV-2 RNA 产生，其 50%有效浓度（EC）为 0.46±0.04 μM。阿利司匹韦抑制了 SARS-CoV-2 生命周期的进入后步骤。这些结果证明，在 SARS-CoV-2 感染患者中快速进行阿利司匹韦的概念验证阶段 2 试验是合理的。
