Bhadrecha Pooja, Bala Madhu, Khasa Yogender Pal, Arshi Anfal, Singh Joginder, Kumar Manoj
Department of Biotechnology, Mangalmay Group of Institutions, Greater Noida, UP India.
2Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organisation, New Delhi, India.
Physiol Mol Biol Plants. 2020 May;26(5):1075-1085. doi: 10.1007/s12298-020-00778-2. Epub 2020 Mar 19.
L. provides an enormous range of medicinal and nutritional benefits. The significant abilities of this plant to survive in Himalayan high altitudes enticed our study to investigate its rhizosphere. Seventeen rhizobacterial strains were isolated from the rhizospheric soil and plant root nodules, belonging to genus , , , , and , as identified by 16SrRNA sequencing. This varying bacterial population was further examined for the presence of root degrading enzymes pectinase and cellulase, which enable them to intrude the plant roots. Based on the growth and substrate utilization by these rhizobacteria on pectinase screening agar medium and Mandels and Reese agar medium, all the seventeen strains were identified as pectinase and cellulase producing rhizobacteria. The quantitative analysis by DNS method demonstrated varying enzyme activities, spot-lighting the physiological variation in the microbiome. The divergence in the enzyme activities shown by all the strains was analysed statistically, using the software ASSISTAT.
L.具有广泛的药用和营养益处。这种植物在喜马拉雅高海拔地区生存的显著能力促使我们的研究对其根际进行调查。从根际土壤和植物根瘤中分离出17株根际细菌菌株，通过16SrRNA测序鉴定，它们属于 、 、 、 、 和 属。进一步检测了这一不同的细菌群体中是否存在能够侵入植物根系的根降解酶果胶酶和纤维素酶。根据这些根际细菌在果胶酶筛选琼脂培养基和曼德尔与里斯琼脂培养基上的生长情况和底物利用情况，所有17株菌株均被鉴定为产生果胶酶和纤维素酶的根际细菌。通过DNS方法进行的定量分析显示了不同的酶活性，突出了微生物群落的生理差异。使用ASSISTAT软件对所有菌株显示的酶活性差异进行了统计分析。
