Zhou Xue, Tian Lei, Zhang Jianfeng, Ma Lina, Li Xiujun, Tian Chunjie
Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, China.
University of the Chinese Academy of Sciences, Beijing, China.
J Basic Microbiol. 2017 Dec;57(12):1055-1064. doi: 10.1002/jobm.201700312. Epub 2017 Sep 13.
Sea buckthorn (Hippophae rhamnoides L.) is a pioneer plant used for land reclamation and an appropriate material for studying the interactions of symbiotic microorganisms because of its nitrogen-fixing root nodules and mycorrhiza. We used high-throughput sequencing to reveal the diversities and community structures of rhizospheric fungi and their link with nitrogen-fixing Frankia harbored in sea buckthorn collected along an altitude gradient from the Qinghai Tibet Plateau to interior areas. We found that the fungal diversities and compositions varied between different sites. Ascomycota, Basidiomycota, and Zygomycota were the dominant phyla. The distribution of sea buckthorn rhizospheric fungi was driven by both environmental factors and the geographic distance. Among all examined soil characteristics, altitude, AP, and pH were found to have significant (p < 0.05) effect on the rhizospheric fungal community. The rhizospheric fungal communities became more distinct as the distance increased. Moreover, co-inertia analysis identified significant co-structures between Frankia and AMF communities in the rhizosphere of sea buckthorn. We conclude that at the large scale, there are certain linkages between nitrogen-fixing bacteria and the AMF expressed in the distributional pattern.
沙棘（Hippophae rhamnoides L.）是一种用于土地复垦的先锋植物，因其固氮根瘤和菌根，是研究共生微生物相互作用的合适材料。我们利用高通量测序揭示了沿青藏高原到内陆地区海拔梯度采集的沙棘根际真菌的多样性和群落结构，以及它们与固氮弗兰克氏菌的联系。我们发现不同地点的真菌多样性和组成有所不同。子囊菌门、担子菌门和接合菌门是优势门类。沙棘根际真菌的分布受环境因素和地理距离的驱动。在所有检测的土壤特征中，海拔、有效磷和pH值对根际真菌群落有显著（p < 0.05）影响。随着距离增加，根际真菌群落变得更加不同。此外，共惯性分析确定了沙棘根际中弗兰克氏菌和丛枝菌根真菌群落之间的显著共结构。我们得出结论，在大尺度上，固氮细菌和丛枝菌根真菌在分布模式上存在一定联系。
