Pré J, Benlatrèche C
Pathol Biol (Paris). 1977 Mar;25(3):203-5.
A simple accurate and very reproducible procedure for measuring serum iron concentration without precipitating serum proteins is described. In the same time, iron is released from serum transferrin, reduced and determined at 535 mn by incubation in a water bath at 55 degrees for five minutes within a formate buffered system (pH 2,6; i = 0,05) including bathophenanthroline sulfonate. This method yields values very similar to those obtained by that of the International Committee for Standardization in Hematology (ICSH).
本文描述了一种简单、准确且重复性极高的测量血清铁浓度的方法，该方法不会使血清蛋白沉淀。同时，在含有磺酸基邻二氮菲的甲酸盐缓冲体系（pH 2.6；离子强度0.05）中，于55℃水浴孵育5分钟，使血清转铁蛋白中的铁释放出来，还原后在535nm处进行测定。该方法所得结果与国际血液学标准化委员会（ICSH）的方法非常相似。
