Lauber K
J Clin Chem Clin Biochem. 1978 May;16(5):315-6.
Two methods for the assay of serum iron are compared; determination by atomic absorption after deproteinisation, and spectrophotometric determination after liberation of the iron by a detergent, reduction with dithionite, and chelation with bathophenanthroline disulfonate, without deproteinisation.
Regression: y = 0.996 x --0.41 (s: atomic absorption; y: bathophenanthroline method; mumol/1 Fe; r = 0.986. Precision (CV) for a series of identical samples with 14.3 mumol/1: 2.0% for atomic absorption and 1.3% for bathophenanthroline.
比较了两种血清铁测定方法;一种是去蛋白后用原子吸收法测定,另一种是在不进行去蛋白的情况下,用去污剂释放铁、连二亚硫酸盐还原以及与4,7-二苯基-1,10-菲啰啉二磺酸螯合后用分光光度法测定。
回归方程:y = 0.996x - 0.41(s:原子吸收法;y:4,7-二苯基-1,10-菲啰啉法;μmol/L铁;r = 0.986。对于一系列浓度为14.3 μmol/L的相同样品,精密度(CV):原子吸收法为2.0%,4,7-二苯基-1,10-菲啰啉法为1.3%。