LEAF Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, University of Lisbon, Lisboa, Portugal; Luxembourg Institute of Science and Technology (LIST), Green Tech platform, Environmental Research and Innovation Department (ERIN), L-4422 Belvaux, Luxembourg.
Luxembourg Institute of Science and Technology (LIST), Green Tech platform, Environmental Research and Innovation Department (ERIN), L-4422 Belvaux, Luxembourg.
J Proteomics. 2020 Jul 15;223:103798. doi: 10.1016/j.jprot.2020.103798. Epub 2020 May 4.
Cistus ladanifer L. is a common shrub endemic to the Mediterranean region with high levels of condensed tannins (CT). CT form complexes with dietary protein resisting microbial degradation in the rumen, which enhances dietary protein utilization in ruminant diets. The objective of this study was to evaluate the utilization of CT in the diet of lambs on the proteomes of muscle, hepatic and adipose tissues. Twenty-four Merino Branco ram lambs were randomly allocated to three treatments (n = 8): C - control (160 g crude protein (CP)) per kg DM, RP - reduced protein (120 g CP/kg DM); and RPCT - reduced protein (120 g CP/kg DM) treated with CT extract. At the end of the trial, lambs were slaughtered and the longissimus lumborum muscle, hepatic and peri-renal adipose tissues sampled. A two-way approach was used for proteomic analysis: 2D-DIGE and nanoLC-MS. In the muscle, C lambs had lower abundance proteins that partake in the glycolysis pathway than the lambs of other treatments. Control lambs had lower abundance of Fe-carrying proteins in the hepatic tissue than RP and RPCT lambs. The latter lambs had highest abundance of hepatic flavin reductase. In the adipose tissue, C lambs had lowest abundance of fatty-acid synthase. SIGNIFICANCE: soybean meal is an expensive feedstuff in which intensive animal production systems heavily rely on. It is a source of protein extensively degraded in the rumen, leading to efficiency losses on dietary protein utilization during digestion. Protection of dietary protein from extensive ruminal degradation throughout the use of plants or extracts rich in CT allow an increase in the digestive utilization of feed proteins. In addition to enhance the protein digestive utilization, dietary CT may induce other beneficial effects in ruminants such as the improvement of the antioxidant status.
乳香树（Cistus ladanifer L.）是一种常见的地中海地区特有灌木，其叶子含有丰富的单宁（CT）。单宁可与日粮蛋白形成复合物，阻止其在瘤胃中被微生物降解，从而提高反刍动物日粮蛋白的利用率。本研究旨在评估日粮 CT 对绵羊肌肉、肝脏和脂肪组织蛋白质组的利用情况。将 24 只 Merino Branco 公羊随机分为 3 个处理组（n=8）：C-对照组（每千克干物质含有 160 克粗蛋白（CP））、RP-低蛋白组（每千克干物质含有 120 克 CP）和 RPCT-低蛋白组（每千克干物质含有 120 克 CP，并添加 CT 提取物）。试验结束时，屠宰绵羊并采集背最长肌、肝脏和肾周脂肪组织。采用双向方法进行蛋白质组学分析：2D-DIGE 和纳升液相色谱-串联质谱（nanoLC-MS）。在肌肉中，C 组绵羊的糖酵解途径相关蛋白丰度低于其他处理组的绵羊。与 RP 和 RPCT 组绵羊相比，对照组绵羊肝脏组织中的含铁蛋白丰度较低。后两组绵羊肝脏中的黄素还原酶丰度最高。在脂肪组织中，C 组绵羊的脂肪酸合酶丰度最低。意义：豆粕是一种昂贵的饲料原料，集约化动物生产系统严重依赖于豆粕。豆粕是一种在瘤胃中广泛降解的蛋白质来源，导致在消化过程中利用日粮蛋白时出现效率损失。通过使用富含 CT 的植物或提取物保护日粮蛋白免受瘤胃的广泛降解，可以提高饲料蛋白的消化利用率。此外，日粮 CT 除了可以提高蛋白消化利用率，还可能对反刍动物产生其他有益影响，如改善抗氧化状态。
