Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, 200433, China.
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, 200433, China.
J Pharm Biomed Anal. 2020 Jul 15;186:113278. doi: 10.1016/j.jpba.2020.113278. Epub 2020 Apr 3.
Tea polyphenols (TP) are the major antioxidant components from tea, which could be beneficial to oxidative stress injury, such as sulfur mustard (SM) exposure. However, the holistic efficacy of TP on SM poisoning remains unexplored and needs further investigation. In this study, Nuclear magnetic resonance(NMR)-based metabolomics along with multivariate statistical analysis was used to explore the metabolic alteration after SM injury and the protective mechanism of TP. Thirteen potential plasma biomarkers of SM injury were identified, which primarily related to synthesis of ketone bodies, arginine and proline metabolism, butanoate metabolism and alanine aspartate and glutamate metabolism. After TP pre-treatment, the biomarkers were mostly restored to normal levels, which suggested that TP provided effective protection against SM injury and might play its role by rebalancing disordered metabolism pathways. This work enhanced our comprehension of the metabolic profiling of SM injury and revealed the protective mechanism of TP.
茶多酚（TP）是茶叶中主要的抗氧化成分，对氧化应激损伤如硫芥（SM）暴露具有一定的益处。然而，TP 对 SM 中毒的整体疗效仍未被探索，需要进一步研究。在这项研究中，我们采用基于核磁共振（NMR）的代谢组学结合多变量统计分析方法，探讨 SM 损伤后的代谢变化以及 TP 的保护机制。鉴定出 13 个 SM 损伤的潜在血浆生物标志物，这些标志物主要与酮体、精氨酸和脯氨酸代谢、丁酸盐代谢以及丙氨酸、天冬氨酸和谷氨酸代谢的合成有关。TP 预处理后，这些生物标志物大多恢复到正常水平，这表明 TP 对 SM 损伤提供了有效的保护作用，可能通过重新平衡紊乱的代谢途径发挥作用。本工作增强了我们对 SM 损伤代谢谱的理解，并揭示了 TP 的保护机制。
