Nobakht B Fatemeh, Arefi Oskouie Afsaneh, Rezaei-Tavirani Mostafa, Aliannejad Rasoul, Taheri Salman, Fathi Fariba, Taghi Naseri Mohammad
a Proteomics Research Center, Faculty of Paramedical Sciences , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
b Department of Basic Sciences, Faculty of Paramedical Sciences , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
Biomarkers. 2017 Jul;22(5):413-419. doi: 10.1080/1354750X.2016.1203995. Epub 2016 Jul 18.
Sulfur mustard (SM) is a vesication chemical warfare agent for which there is currently no antidote. Despite years of research, there is no common consensus about the pathophysiological basis of chronic pulmonary disease caused by this chemical warfare agent. In this study, we combined chemometric techniques with nuclear magnetic resonance (NMR) spectroscopy to explore the metabolic profile of sera from SM-exposed patients. A total of 29 serum samples obtained from 17 SM-injured patients, and 12 healthy controls were analyzed by Random Forest. Increased concentrations of seven amino acids, glycerol, dimethylamine, ketone bodies, lactate, acetate, citrulline and creatine together with the decreased very low-density lipoproteins (VLDL) levels were observed in patients compared with control subjects. Our study reveals the metabolic profile of sera from SM-injured patients and indicates that NMR-based methods can distinguish these patients from healthy controls.
硫芥气（SM）是一种起疱性化学战剂，目前尚无解毒剂。尽管经过多年研究，但对于这种化学战剂所致慢性肺部疾病的病理生理基础仍未达成共识。在本研究中，我们将化学计量学技术与核磁共振（NMR）光谱相结合，以探索硫芥气暴露患者血清的代谢谱。通过随机森林分析了从17名硫芥气中毒患者和12名健康对照者获得的总共29份血清样本。与对照受试者相比，患者中观察到七种氨基酸、甘油、二甲胺、酮体、乳酸、乙酸盐、瓜氨酸和肌酸的浓度升高，同时极低密度脂蛋白（VLDL）水平降低。我们的研究揭示了硫芥气中毒患者血清的代谢谱，并表明基于核磁共振的方法可以将这些患者与健康对照者区分开来。
