Ardali Razieh, Kazemipour Nasrin, Nazifi Saeed, Bagheri Lankarani Kamran, Razeghian Jahromi Iman, Sepehrimanesh Masood
Biochemistry Division, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Clinical Pathology Division, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Intest Res. 2020 Oct;18(4):421-429. doi: 10.5217/ir.2019.00120. Epub 2020 May 8.
BACKGROUND/AIMS: Ulcerative colitis (UC), along with Crohn's disease, is one of the main types of inflammatory bowel disease (IBD). On the other hand, deregulated autophagy is involved in many chronic diseases, including IBD. In this study, we aimed to investigate the role of Atg5 and microRNA-181a (miR-181a) in the pathophysiology of UC.
Colon biopsy, stool, and blood samples of 6 men and 9 women were confirmed for UC. Also, 13 men and 17 women were selected as healthy control (HC). Enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were used to measure the Atg-5 content of the colon biopsies. Besides, the serum and stool levels of Atg5 were measured using ELISA. Moreover, the total RNA of blood cells was extracted and evaluated for the expression of miR-181a.
We found 1.2 ng/mL versus 0.46 ng/mL, 0.34 ng/mL versus 0.24 ng/mL, and 0.082 ng/mL versus 0.062 ng/mL of Atg5 in stool, intestinal tissue, and serum of UC and HCs, respectively. There was no significant difference in the expression of miR-181a in the blood samples of UC and HCs. Immunohistochemistry showed high positivity without any significant difference between the 2 groups in the quantitative analysis.
The significant difference observed between the stool Atg5 content of the HCs and UC patients may provide new insight into using this protein as a diagnostic biomarker, however, considering the small size of our studied population further studies are needed.
背景/目的：溃疡性结肠炎（UC）与克罗恩病一样，是炎症性肠病（IBD）的主要类型之一。另一方面，自噬失调参与包括IBD在内的许多慢性疾病。在本研究中，我们旨在探讨自噬相关基因5（Atg5）和微小RNA-181a（miR-181a）在UC病理生理学中的作用。
对6名男性和9名女性的结肠活检组织、粪便和血液样本进行UC确诊。另外，选取13名男性和17名女性作为健康对照（HC）。采用酶联免疫吸附测定（ELISA）和免疫组织化学法检测结肠活检组织中Atg-5的含量。此外，用ELISA检测血清和粪便中Atg5的水平。而且，提取血细胞的总RNA并评估miR-181a的表达。
我们发现，UC患者和健康对照者粪便中Atg5含量分别为1.2 ng/mL和0.46 ng/mL，肠道组织中分别为0.34 ng/mL和0.24 ng/mL，血清中分别为0.082 ng/mL和0.062 ng/mL。UC患者和健康对照者血液样本中miR-181a的表达无显著差异。免疫组织化学显示高阳性，两组在定量分析中无显著差异。
健康对照者和UC患者粪便中Atg5含量的显著差异可能为将该蛋白用作诊断生物标志物提供新的见解，然而，鉴于我们研究人群规模较小，还需要进一步研究。
