Xu Huihui, Hao Zhongping, Wang Lifang, Li Shuangjiao, Guo Yuruo, Dang Xiangli
Key Laboratory of Biology and Green Control of Plant Diseases and Insect Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China.
Crop Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
Insects. 2020 May 5;11(5):281. doi: 10.3390/insects11050281.
Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a () from was identified and characterized. The consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric point of 7.18. Only a single iron domain was predicted in the N-lobe of PxTrf. Although was expressed ubiquitously, the highest levels of expression were observed in the fourth instar larvae. transcript level was highest in fat bodies among various tissues. The transcript levels increased significantly after the stimulation of pathogens. A decrease in expression via RNA interference enhanced the susceptibility of to the fungus and inhibited hemocyte nodulation in response to the fungal challenge. In addition, a considerable increase in the pupation rate was observed in larvae treated with double-stranded PxTrf (dsPxTrf). Overall, according to the results, PxTrf may participate in immunity against fungal infection and insect development.
转铁蛋白（Trfs）是在铁运输中具有关键功能的多功能蛋白质。在本研究中，从[具体来源]鉴定并表征了一种[具体名称]。该[具体名称]由一个2046 bp的开放阅读框组成，其编码一个分子量为73.43 kDa的681个氨基酸的蛋白质，等电点为7.18。在PxTrf的N叶中仅预测到一个单一的铁结构域。尽管[具体名称]在各处均有表达，但在四龄幼虫中观察到最高表达水平。在各种组织中，[具体名称]转录水平在脂肪体中最高。在病原体刺激后，[具体名称]转录水平显著增加。通过RNA干扰降低[具体名称]表达会增强[具体昆虫名称]对[具体真菌名称]真菌的易感性，并抑制血细胞对真菌攻击的结瘤反应。此外，在用双链PxTrf（dsPxTrf）处理的幼虫中观察到化蛹率显著增加。总体而言，根据结果，PxTrf可能参与[具体昆虫名称]对真菌感染的免疫和昆虫发育。
