Garner Bianca, Brown Elrica, Taplin Martha, Garcia Angel, Williams-Mapp Baracka
Tougaloo College, 500 West County Line Road, Tougaloo, MS, USA.
Tougaloo College, 500 West County Line Road, Tougaloo, MS, USA; Jackson State University, 1400 John R. Lynch Street, Jackson, MS, USA.
Biomed Res Int. 2016;2016:3628268. doi: 10.1155/2016/3628268. Epub 2016 Nov 29.
The present study examined the impact of transferrin on biofilms. Three commercial strains, an environmental strain (33679), the type strain (10792), and an isolate from a diseased insect (700872), were cultured in iron restricted minimal medium. All strains produced biofilm when grown in vinyl plates at 30°C. 33679 had a biofilm biomass more than twice the concentration exhibited by the other strains. The addition of transferrin resulted in slightly increased growth yields for 2 of the 3 strains tested, including 33679. In contrast, the addition of 50 g/mL of transferrin resulted in an 80% decrease in biofilm levels for strain 33679. When the growth temperature was increased to 37°C, the addition of 50 g/mL of transferrin increased culture turbidity for only strain 33679. Biofilm levels were again decreased in strain 33679 at 37°C. Growth of cultures in polystyrene resulted in a decrease in overall growth yields at 30°C, with biofilm levels significantly decreased for 33679 in the presence of transferrin. These findings demonstrate that transferrin impacts biofilm formation in select strains of . Identification of these differences in biofilm regulation may be beneficial in elucidating potential virulence mechanisms among the differing strains.
本研究考察了转铁蛋白对生物膜的影响。三种商业菌株、一种环境菌株（33679）、模式菌株（10792）以及从患病昆虫中分离出的菌株（700872）在缺铁基本培养基中培养。所有菌株在30°C的乙烯基平板上生长时均产生生物膜。33679的生物膜生物量比其他菌株所呈现的浓度高出两倍多。添加转铁蛋白后，测试的3种菌株中有2种（包括33679）的生长产量略有增加。相比之下，添加50μg/mL的转铁蛋白会使33679菌株的生物膜水平降低80%。当生长温度升至37°C时，添加50μg/mL的转铁蛋白仅使33679菌株的培养物浊度增加。在37°C时，33679菌株的生物膜水平再次降低。在聚苯乙烯中培养时，30°C下培养物的总体生长产量下降，在转铁蛋白存在的情况下，33679的生物膜水平显著降低。这些发现表明转铁蛋白会影响特定菌株中生物膜的形成。识别生物膜调控中的这些差异可能有助于阐明不同菌株之间潜在的毒力机制。
