Department of Materials, Imperial College London, London SW7 2AZ, UK.
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan.
Int J Mol Sci. 2020 May 5;21(9):3273. doi: 10.3390/ijms21093273.
Various factors leads to cancer; among them oxidative damage is believed to play an important role. Moreover, it is important to identify a method to detect the oxidative damage. Recently, electrochemical sensors have been considered as the one of the most important techniques to detect DNA damage, owing to its rapid detection. However, electrode materials play an important role in the properties of electrochemical sensor. Currently, researchers have aimed to develop novel electrode materials for low-level detection of biomarkers. Herein, we report the facile hydrothermal synthesis of NiCoO micro flowers (MFs) and NiCoS micro spheres (Ms) and evaluate their electrochemical properties for the detection of carcinogen-causing biomarker 4-nitroquinoline n-oxide (4-NQO) in human blood serum and saliva samples. Moreover, as-prepared composites were fabricated on a glass carbon electrode (GCE), and its electrochemical activities for the determination of 4-NQO were investigated by using various electrochemical techniques. Fascinatingly, the NiCoS-Ms showed a very low detection limit of 2.29 nM and a wider range of 0.005 to 596.64 µM for detecting 4-NQO. Finally, the practical applicability of NiCoS-Ms in the 4-NQO spiked human blood serum and saliva samples were also investigated.
各种因素导致癌症；其中氧化损伤被认为起着重要作用。此外，确定一种检测氧化损伤的方法很重要。最近，由于其快速检测，电化学传感器已被认为是检测 DNA 损伤的最重要技术之一。然而，电极材料在电化学传感器的性能中起着重要作用。目前，研究人员旨在开发新型电极材料，用于低水平检测生物标志物。在此，我们报告了 NiCoO 微花（MFs）和 NiCoS 微球（Ms）的简便水热合成，并评估了它们在人血清和唾液样本中检测致癌生物标志物 4-硝基喹啉 N-氧化物（4-NQO）的电化学性能。此外，将制备的复合材料构建在玻碳电极（GCE）上，并通过各种电化学技术研究了其对 4-NQO 的测定的电化学活性。有趣的是，NiCoS-Ms 对检测 4-NQO 的检测限低至 2.29 nM，检测范围为 0.005 至 596.64 μM。最后，还研究了 NiCoS-Ms 在 4-NQO 掺杂人血清和唾液样本中的实际适用性。
