Lambin P, Dubarry M, Charron C, Herance N, Defendini E
Laboratoire d'Immunochimie, Institut National de Transfusion Sanguine, Paris, France.
Rev Fr Transfus Immunohematol. 1988 Dec;31(5):687-96. doi: 10.1016/s0338-4535(88)80077-1.
We developed a enzyme linked immunosorbent assay (ELISA) for measuring IgG subclasses concentration in serum. For this we used monoclonal antibodies. The specificity of these antibodies was evaluated with a panel of myeloma proteins belonging to the 4 IgG subclasses. The ELISA was sensitive (allowing the detection of subclasses at ng level) and accurate (inter-assay coefficient of variation of 14%). Using the WHO serum 67/97 as reference, we determined the concentration of IgG subclasses in a pool of sera. In addition concentrations were measured in 69 healthy adults to study the distribution of each IgG subclass. A good correlation (r = 0.78) was obtained between the sum of the subclasses measured by ELISA and total IgG measured by immunonephelometry.
Zotero 插件