Kuppers R C, Outschoorn I M, Hamilton R G, Burek C L, Rose N R
Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205.
Clin Immunol Immunopathol. 1993 Apr;67(1):68-77. doi: 10.1006/clin.1993.1046.
A quantitative enzyme-linked immunoassay that measures in absolute terms the subclass concentration of human thyroglobulin (huTg)-specific IgG autoantibody was developed. Unique to this study was the use of an affinity-purified anti-huTg standard with a known concentration of the four IgG subclasses. The sensitivity of the ELISA assay was 1-5 ng/ml depending on the IgG subclass being measured. We examined 22 sera of patients with autoimmune thyroid disease. The total huTg-specific antibody concentrations in serum ranged from 0 to nearly 3000 micrograms/ml of IgG. The IgG subclass distribution in individuals with low huTg-specific IgG (< 10 micrograms/ml) was primarily IgG1 and IgG3 Ab. Patients with intermediate levels of huTg IgG (10-600 micrograms/ml) expressed all four subclasses; however, no particular subclass was dominant. Individuals with > 1000 micrograms/ml also showed huTg-Ab in all four subclasses, however, IgG1 and IgG2 were dominant. All four IgG subclasses were used in the response to huTg, although the pattern of usage varied between individuals. There was no dominant subclass usage seen in this patient population.
我们开发了一种定量酶联免疫测定法，用于绝对测量人甲状腺球蛋白（huTg）特异性IgG自身抗体的亚类浓度。本研究的独特之处在于使用了一种亲和纯化的抗huTg标准品，其四种IgG亚类的浓度已知。根据所检测的IgG亚类不同，ELISA测定法的灵敏度为1-5 ng/ml。我们检测了22例自身免疫性甲状腺疾病患者的血清。血清中总的huTg特异性抗体浓度范围为0至近3000微克/ml IgG。huTg特异性IgG水平较低（<10微克/ml）的个体中，IgG亚类分布主要为IgG1和IgG3抗体。huTg IgG水平中等（10-600微克/ml）的患者表达所有四种亚类；然而，没有特定的亚类占主导。huTg IgG>1000微克/ml的个体也显示所有四种亚类中都有huTg抗体，不过IgG1和IgG2占主导。所有四种IgG亚类都参与了对huTg的反应，尽管个体之间的使用模式有所不同。在该患者群体中未观察到占主导的亚类使用情况。
