Jol-Van der Zijde C M, Labadie J, Vlug A, Radl J, Vossen J M, Van Tol M J
Department of Pediatrics, University Hospital, Leiden, The Netherlands.
J Immunol Methods. 1988 Apr 6;108(1-2):195-203. doi: 10.1016/0022-1759(88)90419-x.
A dot-immunobinding assay on nitrocellulose membranes has been developed for the quantification of human IgG subclasses using subclass-specific monoclonal antibodies. The advantages of this technique can be summarized as follows: (1) possibility of rapid semi-quantitative evaluation and/or precise quantitation from the same dot-pattern; (2) simple procedure with very good reproducibility; (3) sensitivity for nanogram concentrations of individual subclasses, therefore applicable not only to serum but also to other body fluids with a low content of IgG; (4) very small amounts of test material needed; (5) very good correlation of results with other techniques (ELISA, radial immunodiffusion) but without some of the inherent problems of the latter methods.
已开发出一种用于硝酸纤维素膜的斑点免疫结合测定法，以使用亚类特异性单克隆抗体对人IgG亚类进行定量。该技术的优点可总结如下：（1）可从同一斑点模式进行快速半定量评估和/或精确定量；（2）操作简单，重现性好；（3）对纳克浓度的各个亚类具有敏感性，因此不仅适用于血清，也适用于IgG含量低的其他体液；（4）所需测试材料量非常少；（5）结果与其他技术（ELISA、放射免疫扩散）具有很好的相关性，但没有后一种方法的一些固有问题。
