Su Harun Reşid, Turhan Özge, Erman Daloğlu Cemile Aylin, Öğünç Meral Dilara, Özhak Betil, Öngüt Gözde, Kuşkucu Mert Ahmet, Midilli Kenan, Mamıkoğlu Latife
Tokat Hospital, Tokat, Turkey.
Department of Infectious Disease and Clinical Microbiology, University School of Medicine, Akdeniz University, Antalya, Turkey.
Lab Med. 2020 Nov 2;51(6):601-605. doi: 10.1093/labmed/lmaa017.
The aim of this study was to investigate the prevalence of carbapenemase and CTX-M genes among 330 blood culture isolates of Enterobacterales with reduced susceptibility to at least 1 carbapenem, between 2010 and 2015.
BD Max CRE assay and in-house PCR were used to detect carbapenemase and CTX-M genes.
At least 1 carbapenemase gene was detected among 113 (74.3%) of the 152 carbapenem resistant isolates. The OXA-48 (69.7%) was the most common carbapenemase followed by VIM, NDM and IMP, whereas no tested isolates were KPC-positive. Eighty-six isolates (56.6%) had CTX-M and 65 had both OXA-48 and CTX-M. Carbapenemase production in Enterobacterales was significantly increased in years (P < .05).
Our study indicates that there is ongoing endemic circulation of the OXA-48 producing organism in our facility. It is noteworthy that more than half of the OXA-48 producing strains also produced CTX-M enzyme.
本研究旨在调查2010年至2015年间330株对至少1种碳青霉烯类药物敏感性降低的肠杆菌科血培养分离株中碳青霉烯酶和CTX-M基因的流行情况。
采用BD Max CRE检测法和内部聚合酶链反应（PCR）检测碳青霉烯酶和CTX-M基因。
在152株耐碳青霉烯类菌株中，113株（74.3%）检测到至少1种碳青霉烯酶基因。最常见的碳青霉烯酶是OXA-48（69.7%），其次是VIM、NDM和IMP，而检测的分离株中没有KPC阳性的。86株（56.6%）有CTX-M基因，65株同时有OXA-48和CTX-M基因。肠杆菌科中碳青霉烯酶的产生在各年份显著增加（P <.05）。
我们的研究表明，在我们的机构中，产OXA-48的生物体正在持续地方性传播。值得注意的是，超过一半的产OXA-48菌株也产生CTX-M酶。
