Carbapenem-resistant (CRE) have been responsible for nosocomial outbreaks worldwide and have become endemic in several countries. To better understand the epidemiological trends and characteristics of CRE in the Henan province. We assessed the molecular epidemiological characteristics of 305 CRE strains isolated from patients in 19 secondary or tertiary hospitals in ten areas of the Henan province in China. A total of 305 CRE isolates were subjected to multiple tests, including antimicrobial susceptibility testing, PCR for carbapenemase genes , , , , -. Tigecycline-resistant genes , , , A, , X, M, L were analysed in five tigecycline non-susceptible carbapenem-resistant isolates (TNSCRKP). Additionally, multilocus sequence typing (MLST) was performed for carbapenem-resistant (CRKP). The most common CRE species were (234, 77 %), (36, 12 %) and (13, 4 %). All strains exhibited multi-drug resistance. Overall, 97 % (295/305) and 97 % (297/305) of the isolates were susceptible to polymyxin B and tigecycline, respectively. A total of 89 % (271/305) of the CRE isolates were carbapenemase gene-positive, including 70 % , 13 % , 6 % , and 1 % combined / genes. carbapenemase (KPC) was the predominant carbapenemase in (87 %), whereas NDM and IMP were frequent in (53 %) and (69 %), respectively. Mutations in the , and genes were detected in five TNSCRKP. Moreover, 15 unique sequence types were detected, with ST11 (74 %), ST15 (9 %) and ST2237 (5 %) being dominant among strains. A high proportion of CRE strains were carbapenemase-positive, and five carbapenem-resistant isolates were tigecycline non-susceptible, indicating a need for the ongoing surveillance of CRE and effective measures for the prevention of CRE infections.