Hsu W T, Harvey R G, Lin E J, Weiss S B
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1378-82. doi: 10.1073/pnas.74.4.1378.
The usefulness of bacterial viruses for detecting substances that are potentially carcinogenic is reexamined as a model system for screening biologically active polycyclic aromatic hydrocarbons. A modification of the original assay procedure allows one to distinguish between aromatics that can modify the biological activity of infectious nucleic acids directly and those polycyclic aromatic hydrocarbons that require metabolic activation by Escherichia coli enzymes. The effect of chemical modification of several different polycyclic aromatic hydrocarbons, with respect to their biological activity in the phage assay system, is described. Among the 31 different compounds examined, (+/-)-anti-benzo[a]pyrene-7,8-diol-9,10-epoxide was the most potent inhibitor of infectious phage nucleic acid. The (+) and (-) isomers of the above racemic mixture did not differ significantly in their capacity to inhibit phage replication.
作为筛选具有生物活性的多环芳烃的模型系统，对细菌病毒用于检测潜在致癌物质的实用性进行了重新审视。对原始检测程序的改进使人们能够区分可直接改变感染性核酸生物活性的芳烃与那些需要大肠杆菌酶进行代谢激活的多环芳烃。描述了几种不同多环芳烃的化学修饰对其在噬菌体检测系统中的生物活性的影响。在所检测的31种不同化合物中，(±)-反式苯并[a]芘-7,8-二醇-9,10-环氧化物是感染性噬菌体核酸的最有效抑制剂。上述外消旋混合物的(+)和(-)异构体在抑制噬菌体复制的能力上没有显著差异。
