Ames B N, Lee F D, Durston W E
Proc Natl Acad Sci U S A. 1973 Mar;70(3):782-6. doi: 10.1073/pnas.70.3.782.
We previously described a set of four strains of Salmonella typhimurium designed for detecting the various types of mutagens, and showed their utility in detecting a wide variety of carcinogens as mutagens. The lipopolysaccharide that normally coats these bacteria is a barrier to penetration of mutagens to the cell membrane. The set of tester strains has been improved by adding a mutation (rfa: deep rough) that results in a deficient lipopolysaccharide. The techniques for using these strains for detecting mutagens are presented and the tests are shown to be extremely sensitive and convenient. The specificity of frameshift mutagenesis is clarified. As adjuncts to the test with the four strains, we describe a test that compares mutagenic killing in deep rough strains with and without DNA excision repair, and a test using forward mutagenesis in a deep rough strain lacking excision repair.
我们之前描述了一组用于检测各种类型诱变剂的鼠伤寒沙门氏菌菌株，并展示了它们在检测多种致癌物作为诱变剂方面的实用性。通常覆盖这些细菌的脂多糖是诱变剂穿透细胞膜的障碍。通过添加一个导致脂多糖缺陷的突变（rfa：深粗糙型），对这组测试菌株进行了改进。介绍了使用这些菌株检测诱变剂的技术，并且这些测试被证明极其灵敏且方便。明确了移码诱变的特异性。作为使用这四种菌株进行测试的辅助手段，我们描述了一种比较有和没有DNA切除修复的深粗糙型菌株中诱变杀伤作用的测试，以及一种在缺乏切除修复的深粗糙型菌株中使用正向诱变的测试。
