苯并(a)芘-7,8-二氢二醇-9,10-环氧化物使噬菌体phiX174 DNA失活的机制
Mechanism of phage phiX174 DNA inactivation by benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide.
作者信息
Hsu W T, Lin E J, Harvey R G, Weiss S B
出版信息
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3335-9. doi: 10.1073/pnas.74.8.3335.
A previous report from this laboratory has shown that certain derivatives of polycyclic aromatic hydrocarbons bind to phiX174 DNA and render it noninfectious. The present work describes the relationship between the extent of phiX174 DNA binding by (+/-)-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide and the effect on infectivity. The results suggest that one molecule of bound diolepoxide is sufficient to inhibit the replication of a single molecule of phiX174 DNA. DNA synthesis studies, in vitro, indicate that when phiX DNA bound by benzo[a]pyrene groups serves as template the rate of DNA polymerization is reduced and less product is formed. In addition, the propagation of synthetic DNA strands is blocked so that incomplete complementary chains are assembled. The relationship of these findings to the mutagenic and carcinogenic process associated with the action of benzo[a]pyrene-diolepoxide is discussed.
该实验室之前的一份报告显示，多环芳烃的某些衍生物会与φX174 DNA结合并使其失去感染性。目前的工作描述了（±）-反式苯并[a]芘-7,8-二氢二醇-9,10-环氧化物与φX174 DNA的结合程度及其对感染性的影响之间的关系。结果表明，一个结合的二环氧物分子足以抑制单个φX174 DNA分子的复制。体外DNA合成研究表明，当被苯并[a]芘基团结合的φX DNA作为模板时，DNA聚合速率降低且形成的产物较少。此外，合成DNA链的延伸受阻，从而组装成不完整的互补链。讨论了这些发现与苯并[a]芘二环氧物作用相关的诱变和致癌过程的关系。
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