大肠杆菌对亮氨酰转运RNA的差异利用
Differential utilization of leucyl-tRNAs by Escherichia coli.
作者信息
Holmes W M, Goldman E, Miner T A, Hatfield G W
出版信息
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1393-7. doi: 10.1073/pnas.74.4.1393.
Abstract
The utilization of the isoaccepting species of leucyl-tRNA in protein synthesis has been examined in E. coli. Two minor leucyl-tRNA species, isoacceptors tRNA3Leu and tRNA4Leu, are the predominant species found bound to ribosomes during exponential growth in minimal medium. In rich medium, an increased proportion of tRNA1Leu is found bound to ribosomes. One species, tRNA5Leu, is always absent from ribosomes. In a mutant strain in which normal tRNA3Leu and tRNA5Leu are reduced or absent, tRNA1Leu is a major ribosome-bound species in minimal medium. Protein synthesis in vitro with RNA from E. coli further suggests that tRNA5Leu is rarely used for total protein synthesis; however, this species is active with RNA from MS2 phage. We propose that tRNA1Leu can substitute for tRNA3Leu under rapid growth conditions, and that tRNA5Leu is used minimally in total protein synthesis.
摘要
已在大肠杆菌中研究了亮氨酰 - tRNA的同功受体种类在蛋白质合成中的利用情况。两种次要的亮氨酰 - tRNA种类，同功受体tRNA3Leu和tRNA4Leu，是在基本培养基中指数生长期间发现与核糖体结合的主要种类。在丰富培养基中，发现与核糖体结合的tRNA1Leu比例增加。有一种tRNA5Leu种类，在核糖体中始终不存在。在一个突变菌株中，正常的tRNA3Leu和tRNA5Leu减少或缺失，tRNA1Leu是基本培养基中与核糖体结合的主要种类。用大肠杆菌的RNA进行体外蛋白质合成进一步表明，tRNA5Leu很少用于总蛋白质合成；然而，该种类对MS2噬菌体的RNA有活性。我们提出，在快速生长条件下，tRNA1Leu可以替代tRNA3Leu，并且tRNA5Leu在总蛋白质合成中的使用最少。
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