亮氨酸tRNA与噬菌体T2感染后大肠杆菌蛋白质合成的终止
Leucine tRNA and cessation of Escherichia coli protein synthesis upon phage T2 infection.
作者信息
Kano-Sueoka T, Sueoka N
出版信息
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1229-36. doi: 10.1073/pnas.62.4.1229.
Abstract
The involvement of tRNA in cellular differentiation has been tested by analyzing aminoacyl-tRNA of Escherichia coli after phage T2 infection. One or two minutes after infection, half of one of the five leucine tRNA components (Leu-tRNA(1), CUG responding) undergoes a drastic structural change which leads to inactivity of both leucine acceptor activity and codon response. Whether or not the modification causes cessation of host protein synthesis without inhibiting phage-specific protein synthesis has been examined by analyzing polysome-bound leucine tRNA of E. coli before and after the phage infection. The results presented in this paper indicate that the amount of Leu-tRNA(1) used after infection was greatly reduced as compared to that used in noninfected cells. Studies of the in vitro protein-synthesizing system show that T2 mRNA rarely contains the CUG codon. A mechanism by which host mRNA translation is inhibited by the phage infection is proposed from this available information.
摘要
通过分析噬菌体T2感染后大肠杆菌的氨酰tRNA，对tRNA参与细胞分化进行了测试。感染后一到两分钟，五种亮氨酸tRNA成分之一（Leu-tRNA(1)，识别密码子CUG）的一半发生剧烈的结构变化，导致亮氨酸接受活性和密码子反应均失活。通过分析噬菌体感染前后大肠杆菌多核糖体结合的亮氨酸tRNA，研究了这种修饰是否会在不抑制噬菌体特异性蛋白质合成的情况下导致宿主蛋白质合成停止。本文给出的结果表明，与未感染细胞相比，感染后使用的Leu-tRNA(1)数量大幅减少。体外蛋白质合成系统研究表明，T2 mRNA很少包含CUG密码子。根据这些现有信息，提出了一种噬菌体感染抑制宿主mRNA翻译的机制。
相似文献
1
Leucine tRNA and cessation of Escherichia coli protein synthesis upon phage T2 infection.亮氨酸tRNA与噬菌体T2感染后大肠杆菌蛋白质合成的终止
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1229-36. doi: 10.1073/pnas.62.4.1229.
2
Effect of bacteriophage ghost infection on protein synthesis in Escherichia coli.噬菌体幽灵感染对大肠杆菌蛋白质合成的影响。
J Virol. 1972 Oct;10(4):713-20. doi: 10.1128/JVI.10.4.713-720.1972.
3
Patterns of E. coli leucine tRNA isoacceptors following bacteriophage MS2 infection.噬菌体MS2感染后大肠杆菌亮氨酸tRNA同工受体的模式。
Nucleic Acids Res. 1976 Apr;3(4):917-30. doi: 10.1093/nar/3.4.917.
4
Mechanisms in protein synthesis. XIV. Competitive inhibition of natural mRNA-stimulated amino acid incorporation by aminoacyl-tRNA.蛋白质合成机制。十四。氨酰-tRNA对天然mRNA刺激的氨基酸掺入的竞争性抑制。
Biochim Biophys Acta. 1973 Mar 28;299(3):468-71. doi: 10.1016/0005-2787(73)90271-2.
5
The early change in E. coli leucine tRNA after infection with bacteriophage T2.感染噬菌体T2后大肠杆菌亮氨酸tRNA的早期变化。
Biochem Biophys Res Commun. 1968 Sep 30;32(6):971-6. doi: 10.1016/0006-291x(68)90123-x.
6
Differential utilization of leucyl-tRNAs by Escherichia coli.大肠杆菌对亮氨酰转运RNA的差异利用
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1393-7. doi: 10.1073/pnas.74.4.1393.
7
Coding specificity of Escherichia coli leucine transfer ribonucleic acids and effect of bacteriophage T2 infection.大肠杆菌亮氨酸转移核糖核酸的编码特异性及噬菌体T2感染的影响。
J Mol Biol. 1970 Sep 14;52(2):179-93. doi: 10.1016/0022-2836(70)90024-0.
8
Specificity of codon recognition by Escherichia coli tRNALeu isoaccepting species determined by protein synthesis in vitro directed by phage RNA.通过噬菌体RNA体外指导的蛋白质合成确定大肠杆菌亮氨酰tRNA同工受体种类对密码子识别的特异性。
J Mol Biol. 1979 Apr 25;129(4):567-85. doi: 10.1016/0022-2836(79)90469-8.
9
Transfer RNA and aminoacyl-tRNA synthetases in cells of E. coli infected with phage MS2.感染噬菌体MS2的大肠杆菌细胞中的转运RNA和氨酰-tRNA合成酶
Mol Biol. 1972 Nov-Dec;6(6):674-8.
10
The effect of tRNA concentration on the rate of protein synthesis.转运RNA浓度对蛋白质合成速率的影响。
Proc Natl Acad Sci U S A. 1969 Feb;62(2):566-73. doi: 10.1073/pnas.62.2.566.
引用本文的文献
1
Specific degradation of a plant leucyl transfer ribonucleic Acid by a factor in the homologous synthetase preparation.植物亮氨酰转移核糖核酸由同源合成酶制剂中的一种因子的特异性降解。
Plant Physiol. 1973 Sep;52(3):292-7. doi: 10.1104/pp.52.3.292.
2
Structure of an Escherichia coli tRNA operon containing linked genes for arginine, histidine, leucine, and proline tRNAs.一个包含精氨酸、组氨酸、亮氨酸和脯氨酸tRNA相关基因的大肠杆菌tRNA操纵子的结构。
J Bacteriol. 1984 Jun;158(3):934-42. doi: 10.1128/jb.158.3.934-942.1984.
3
Inhibition of replication of ribonucleic acid bacteriophage f2 by superinfection with bacteriophage T4.噬菌体T4的超感染对核糖核酸噬菌体f2复制的抑制作用。
J Virol. 1971 Oct;8(4):417-29. doi: 10.1128/JVI.8.4.417-429.1971.
4
Analysis of isoaccepting transfer ribonucleic acid species of Bacillus subtilis: chromatographic differences between transfer ribonucleic acids from spores and cells in exponential growth.枯草芽孢杆菌同功接受转移核糖核酸种类的分析：指数生长期孢子和细胞的转移核糖核酸之间的色谱差异
J Bacteriol. 1973 Feb;113(2):825-33. doi: 10.1128/jb.113.2.825-833.1973.
5
Pleiotropic effects of suppressor mutations in Bacillus subtilis.枯草芽孢杆菌中抑制基因突变的多效性效应。
J Bacteriol. 1974 Sep;119(3):961-75. doi: 10.1128/jb.119.3.961-975.1974.
6
Common evolutionary origin of the phage T4 dam and host Escherichia coli dam DNA-adenine methyltransferase genes.噬菌体T4 dam与宿主大肠杆菌dam DNA-腺嘌呤甲基转移酶基因的共同进化起源。
J Bacteriol. 1985 Nov;164(2):932-7. doi: 10.1128/jb.164.2.932-937.1985.
7
Alteration of tyrosine isoaccepting transfer ribonucleic acid species in wild-type and asporogenous strains of Bacillus subtilis.枯草芽孢杆菌野生型和无芽孢生成菌株中酪氨酸同工受体转移核糖核酸种类的改变。
J Bacteriol. 1975 May;122(2):526-31. doi: 10.1128/jb.122.2.526-531.1975.
8
Shutoff of host macromolecular synthesis after T-even bacteriophage infection.T-偶数噬菌体感染后宿主大分子合成的关闭。
Microbiol Rev. 1979 Jun;43(2):199-223. doi: 10.1128/mr.43.2.199-223.1979.
9
Differential utilization of leucyl-tRNAs by Escherichia coli.大肠杆菌对亮氨酰转运RNA的差异利用
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1393-7. doi: 10.1073/pnas.74.4.1393.
10
In vivo aminoacylation of transfer ribonucleic acid in Bacillus subtilis and evidence for differential utilization of lysine-isoaccepting transfer ribonucleic acid species.枯草芽孢杆菌中转录核糖核酸的体内氨基酰化作用以及赖氨酸同工接受转移核糖核酸种类差异利用的证据。
J Bacteriol. 1977 Jun;130(3):1091-7. doi: 10.1128/jb.130.3.1091-1097.1977.
本文引用的文献
1
A SPECIFIC MODIFICATION OF LEUCYL-SRNA OF ESCHERICHIA COLI AFTER PHAGE T2 INFECTION.噬菌体T2感染后大肠杆菌亮氨酰-sRNA的一种特异性修饰
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1535-40. doi: 10.1073/pnas.52.6.1535.
2
THE BINDING OF S-RNA BY ESCHERICHIA COLI RIBOSOMES.大肠杆菌核糖体与S-RNA的结合
J Mol Biol. 1963 Oct;7:360-78. doi: 10.1016/s0022-2836(63)80030-3.
3
Modification of leucyl-sRNA after bacteriophage infection.噬菌体感染后亮氨酰-sRNA的修饰
J Mol Biol. 1966 Sep;20(1):183-209. doi: 10.1016/0022-2836(66)90124-0.
4
Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis.通过冻融-溶菌酶裂解从大肠杆菌中提取的多核糖体。
Science. 1966 Sep 2;153(3740):1119-20. doi: 10.1126/science.153.3740.1119.
5
Separation of transfer ribonucleic acids by reverse phase chromatography.通过反相色谱法分离转移核糖核酸
J Biol Chem. 1965 Oct;240(10):3979-83.
6
Effect of bacteriophage infection upon the specificity of leucine transfer RNA for RNA codewords.噬菌体感染对亮氨酸转运RNA针对RNA密码子特异性的影响。
J Mol Biol. 1968 Jul 14;35(1):1-12. doi: 10.1016/s0022-2836(68)80033-6.
7
Characterization of a modified leucyl-tRNA of Escherichia coli after bacteriophage T2 infection.噬菌体T2感染后大肠杆菌修饰的亮氨酰tRNA的特性分析。
J Mol Biol. 1968 Nov 14;37(3):475-91. doi: 10.1016/0022-2836(68)90116-2.
8
Characterization of leucine transfer ribonucleic acid in Escherichia coli following infection with bacteriophage T2.噬菌体T2感染后大肠杆菌中亮氨酸转移核糖核酸的特性分析
J Biol Chem. 1968 Nov 10;243(21):5584-90.
9
Transfer RNA coded by the T4 bacteriophage genome.由T4噬菌体基因组编码的转运核糖核酸。
Proc Natl Acad Sci U S A. 1968 Sep;61(1):114-21. doi: 10.1073/pnas.61.1.114.
10
Influence of T4 on the formation of RNA phage-specific polyribosomes and polymerase.T4对RNA噬菌体特异性多核糖体和聚合酶形成的影响。
J Mol Biol. 1968 Aug 14;35(3):513-22. doi: 10.1016/s0022-2836(68)80011-7.
Zotero 插件