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噬菌体MS2感染后大肠杆菌亮氨酸tRNA同工受体的模式。

Patterns of E. coli leucine tRNA isoacceptors following bacteriophage MS2 infection.

作者信息

Di Natale P, Eilat D

出版信息

Nucleic Acids Res. 1976 Apr;3(4):917-30. doi: 10.1093/nar/3.4.917.

Abstract

The RNA extracted from MS2 phage particles can accept radioactive leucine and serine in the presence of tRNA activating enzymes. Leucine acceptance is due to the presence of E. coli leucine tRNA that binds very tightly to the virus particle. RPC-5 column chromatography shows that the pattern of virus associated leucyl-tRNA isoacceptors is different from that of normal E. coli leucyl-tRNA. It is also different from the pattern of host leucyl-tRNA isoacceptors found in E. coli lysate following MS2 phage infection. The RPC-5 pattern of the latter tRNA shows several new peaks of leucine tRNA isoacceptors. The possibility that these tRNAs are some modified forms of normal leucine tRNA isoacceptors is suggested.

摘要

从MS2噬菌体颗粒中提取的RNA在tRNA激活酶存在的情况下能够接受放射性亮氨酸和丝氨酸。亮氨酸的接受是由于存在与病毒颗粒紧密结合的大肠杆菌亮氨酸tRNA。RPC - 5柱色谱显示,与病毒相关的亮氨酰 - tRNA同工受体的模式不同于正常大肠杆菌亮氨酰 - tRNA的模式。它也不同于MS2噬菌体感染后在大肠杆菌裂解物中发现的宿主亮氨酰 - tRNA同工受体的模式。后一种tRNA的RPC - 5模式显示出亮氨酸tRNA同工受体的几个新峰。有人提出这些tRNA可能是正常亮氨酸tRNA同工受体的一些修饰形式。

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Differential utilization of leucyl-tRNAs by Escherichia coli.大肠杆菌对亮氨酰转运RNA的差异利用
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1393-7. doi: 10.1073/pnas.74.4.1393.

本文引用的文献

3
A method for the large-scale preparation of bacteriophage MS2.一种大规模制备噬菌体MS2的方法。
Biochim Biophys Acta. 1965 Sep 6;108(1):142-3. doi: 10.1016/0005-2787(65)90119-x.
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Detection of bacteriophage T4- and T5-coded transfer RNAs.噬菌体T4和T5编码的转移RNA的检测
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1164-71. doi: 10.1073/pnas.67.3.1164.
6
Transfer RNA and cell differentiation.转运RNA与细胞分化
Prog Nucleic Acid Res Mol Biol. 1970;10:23-55. doi: 10.1016/s0079-6603(08)60560-7.

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