光驱动大肠杆菌细胞分裂用于化学物质生产。

Light-powered Escherichia coli cell division for chemical production.

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China.

Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China.

出版信息

Nat Commun. 2020 May 8;11(1):2262. doi: 10.1038/s41467-020-16154-3.

Cell division can perturb the metabolic performance of industrial microbes. The C period of cell division starts from the initiation to the termination of DNA replication, whereas the D period is the bacterial division process. Here, we first shorten the C and D periods of E. coli by controlling the expression of the ribonucleotide reductase NrdAB and division proteins FtsZA through blue light and near-infrared light activation, respectively. It increases the specific surface area to 3.7 μm and acetoin titer to 67.2 g·L. Next, we prolong the C and D periods of E. coli by regulating the expression of the ribonucleotide reductase NrdA and division protein inhibitor SulA through blue light activation-repression and near-infrared (NIR) light activation, respectively. It improves the cell volume to 52.6 μm and poly(lactate-co-3-hydroxybutyrate) titer to 14.31 g·L. Thus, the optogenetic-based cell division regulation strategy can improve the efficiency of microbial cell factories.

细胞分裂会干扰工业微生物的代谢性能。细胞分裂的 C 期始于 DNA 复制的开始到终止，而 D 期是细菌分裂过程。在这里，我们首先通过分别控制蓝、近红外光激活的核糖核苷酸还原酶 NrdAB 和分裂蛋白 FtsZA 的表达来缩短大肠杆菌的 C 和 D 期。这将比表面积提高到 3.7μm，乙酰 3.7μm 产量提高到 67.2g·L-1。接下来，我们通过分别调控蓝、近红外（NIR）光激活抑制的核糖核苷酸还原酶 NrdA 和分裂蛋白抑制剂 SulA 的表达来延长大肠杆菌的 C 和 D 期。这将细胞体积提高到 52.6μm，聚（乳酸-共-3-羟基丁酸）产量提高到 14.31g·L-1。因此，基于光遗传学的细胞分裂调控策略可以提高微生物细胞工厂的效率。