State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
Metab Eng. 2019 Jul;54:127-136. doi: 10.1016/j.ymben.2019.03.010. Epub 2019 Apr 3.
Adaptive evolution is an effective strategy to obtain industrial strains with excellent performance, but this method is time-consuming and less efficient. To solve these problems, we constructed an autonomous evolution mutation system (AEMS) to promote the occurrence of mutations, based on the hierarchical dynamic control between the high-fidelity module and the mutagenic module. AEMS was applied in Bacillus subtilis to screen acetoin tolerance phenotypes and high-producing strains. The minimal inhibitory concentration of acetoin exhibited a large increase from 40 to 60 g/L, and acetoin production was increased by 42.8% compared to that of B. subtilis 168. Scaled-up production of acetoin with the directed evolution strain B. subtilis HS019 in a 30-L fermentor resulted in acetoin titer up to 82.5 g/L. These results indicated that AEMS could improve the efficiency of evolution and provide an effective method for obtaining tolerance phenotypes and high-producing strains.
自适应进化是获得具有优异性能的工业菌株的有效策略，但这种方法耗时且效率较低。为了解决这些问题，我们构建了一个自主进化突变系统（AEMS），基于高保真模块和诱变模块之间的层次动态控制，促进突变的发生。AEMS 被应用于枯草芽孢杆菌中筛选乙酰耐受表型和高产菌株。乙酰的最小抑制浓度从 40 克/升显著增加到 60 克/升，与枯草芽孢杆菌 168 相比，乙酰的产量提高了 42.8%。在 30 升发酵罐中用定向进化菌株枯草芽孢杆菌 HS019 进行乙酰的放大生产，乙酰**的浓度高达 82.5 克/升。这些结果表明，AEMS 可以提高进化效率，为获得耐受表型和高产菌株提供了一种有效方法。
