Department of Biological Sciences, New Jersey Institute of Technology, Newark, NJ, 07102, USA.
Department of Biological Sciences, 132 Long Hall, Clemson University, Clemson, SC, 29634, USA.
Sci Rep. 2020 May 8;10(1):7744. doi: 10.1038/s41598-020-64269-w.
In the marine realm, eusociality is only known to have evolved within a clade of sponge-dwelling snapping shrimps in the genus Synalpheus. Deciphering the genomic underpinnings of eusociality in these marine shrimps has been limited by the sparse genomic resources in this genus. Here, we report, for a eusocial shrimp Synalpheus microneptunus, a complete mitochondrial genome (22X coverage) assembled from short Illumina 150 bp paired-end reads. The 15,603 bp long mitochondrial genome of S. microneptunus is AT-rich and includes 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, 22 transfer RNA genes and an 834 bp intergenic region assumed to be the D-loop. The gene order is identical to that reported for most caridean shrimps and corresponds to the presumed Pancrustacean ground pattern. All PCGs showed signs of purifying selection, with K/K <<1 across the whole PCGs and most sliding windows within PCGs. Maximum-likelihood and Bayesian inference phylogenetic analyses of 13 PCGs and 68 terminals supports the monophyly of the Caridea and the family Alpheidae. The complete mitochondrial genome of the eusocial shrimp Synalpheus microneptunus will contribute to a better understanding of the selective pressures and rates of molecular evolution in marine eusocial animals.
在海洋领域，只有在海绵居住的 snapping 虾属 Synalpheus 的一个分支中，才知道真社会性是如何进化的。由于这个属的基因组资源稀少，因此对这些海洋虾类真社会性的基因组基础的破译一直受到限制。在这里，我们报告了一种真社会性虾类 Synalpheus microneptunus 的完整线粒体基因组（22X 覆盖率），该基因组是通过短的 Illumina 150bp 配对末端读数组装而成的。S. microneptunus 的 15603bp 长线粒体基因组富含 AT，包括 13 个蛋白质编码基因（PCGs）、2 个核糖体 RNA 基因、22 个转移 RNA 基因和一个 834bp 的基因间隔区，假定为 D-loop。基因顺序与大多数 caridean 虾类报告的基因顺序相同，与假定的泛甲壳动物基础模式相对应。所有 PCGs 都显示出纯化选择的迹象，整个 PCGs 以及大多数 PCGs 内的滑动窗口中的 K/K <<1。基于 13 个 PCGs 和 68 个末端的最大似然和贝叶斯推断系统发育分析支持 Caridea 科和 Alpheidae 科的单系性。真社会性虾类 Synalpheus microneptunus 的完整线粒体基因组将有助于更好地理解海洋真社会性动物的选择压力和分子进化率。
