大肠杆菌β-半乳糖苷酶的氨基酸序列。
The amino acid sequence of beta-galactosidase of Escherichia coli.
作者信息
Fowler A V, Zabin I
出版信息
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1507-10. doi: 10.1073/pnas.74.4.1507.
Abstract
The amino acid sequence of beta-galactosidase was determined. The protein contains 1021 amino acid residues in a single polypeptide chain. The subunit molecular weight calculated from the sequence is 116,248. The sequence determination, carried out mainly by conventional methods, was aided by complementation tests, by the use of termination mutant strains, and by a new immunochemical method. The five residue sequence Thr-Pro-His-Pro-Ala appears twice within the polypeptide chain, but no other striking homologous features are evident.
摘要
测定了β-半乳糖苷酶的氨基酸序列。该蛋白质在一条单一的多肽链中含有1021个氨基酸残基。根据序列计算出的亚基分子量为116248。序列测定主要通过传统方法进行，互补试验、使用终止突变菌株以及一种新的免疫化学方法对其起到了辅助作用。五残基序列苏氨酸-脯氨酸-组氨酸-脯氨酸-丙氨酸在多肽链中出现了两次，但没有其他明显的显著同源特征。
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