Fowler A V, Brake A J, Zabin I
J Biol Chem. 1978 Aug 10;253(15):5515-20.
The sequence of the COOH-terminal third (omega) of beta-galactosidase is presented. The size of the 7 cyanogen bromide peptides of this segment is larger on the average, about 52 amino acid residues as compared to an average size of 42 for cyanogen bromide peptides in the whole molecule. Tyrosine, phenylalanine, and valine are low in this segment whereas alanine and lysine are high. This region has a slight excess of basic groups.
本文给出了β-半乳糖苷酶羧基末端三分之一(ω)的序列。该片段的7个溴化氰肽平均尺寸更大,与整个分子中溴化氰肽平均42个氨基酸残基的尺寸相比,约为52个氨基酸残基。该片段中酪氨酸、苯丙氨酸和缬氨酸含量较低,而丙氨酸和赖氨酸含量较高。该区域碱性基团略有过量。