mTc-HMPAO 标记白细胞的细胞分类分析。

Analysis of Cell Fraction of mTc-HMPAO Radiolabeled Leukocytes.

机构信息

Department of Nuclear Medicine, Molecular Imaging and Therapeutic Medicine Research Center, Research Institute of Clinical Medicine of Jeonbuk National University and Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, Jeonbuk, Korea.

出版信息

Curr Radiopharm. 2020;13(2):142-148. doi: 10.2174/1874471013666200510015742.

DOI:10.2174/1874471013666200510015742

PMID:32386504

Abstract

PURPOSE

99mTc-HMPAO radiolabeled autologous leukocyte scintigraphy is routinely used clinically for infection imaging. Leukocytes are mostly separated via sedimentation. It is unknown whether leukocytes are clearly separated by sedimentation or selectively labeled. Therefore, in this study, the blood cell numbers were investigated after leukocyte radiolabeling to identify the cells strongly radiolabeled by 99mTc-HMPAO.

METHODS

This study was performed with leftover blood samples of the patients who underwent 99mTc-HMPAO scintigraphy at Chonbuk National University Hospital (2018-2019). The blood of 22 patients was drawn for 99mTc-HMPAO scintigraphy. WBCs were separated via conventional sedimentation at our clinic and radiolabeled. The concentration of cell components was determined using an automatic hematology analyzer. The cells in the final sample injectate sample were separated using Histopaque and counted with a dose calibrator.

RESULTS

The average numbers of RBCs, WBCs, and PLTs in the final injection sample were 79 ± 33, 23.26 ± 11.95, and 229.5 ± 206.57 x 103/μL, respectively. The PLT number was almost 10-fold the number of WBCs. The number of RBCs was nearly 3-fold higher than WBCs [RBC/WBC ratio = 4.67 ± 3.58, and PLT/WBC ratio = 10.65 ± 12.46]. Following Histopaque separation, the activity of each layer showed 99mTc-HMPAO labeling of WBC > RBC > PLT in order. The total activity/cell numbers of WBCs, RBCs and PLTs were 0.016 ± 0.010, 0.005 ± 0.005 and 0.003 ± 0.002, respectively (p > 0.05).

CONCLUSION

Although the numbers of RBCs and PLTs were highly increased after sedimentation, their individual cellular activity was lower than that of WBCs. 99mTc-HMPAO was more selective to WBCs than RBCs or PLTs. In conclusion, a higher number of WBCs were radiolabeled compared with RBCs and PLTs.

摘要

目的

99mTc-HMPAO 放射性标记自身白细胞闪烁照相术常用于临床感染成像。白细胞主要通过沉降分离。目前尚不清楚白细胞是否通过沉降得到明显分离，还是选择性标记。因此，在这项研究中，我们研究了白细胞放射性标记后血细跑数量的变化，以鉴定被 99mTc-HMPAO 强烈标记的细胞。

方法

本研究使用 2018-2019 年在全北国立大学医院进行 99mTc-HMPAO 闪烁照相术的患者的剩余血液样本进行。我们的诊所通过常规沉降分离患者的血液并进行放射性标记。使用自动血细胞分析仪测定细胞成分的浓度。使用Histopaque 分离最终样品注射器中的细胞，并使用剂量校准器进行计数。

结果

最终注射样品中 RBCs、WBCs 和 PLTs 的平均数量分别为 79±33、23.26±11.95 和 229.5±206.57×103/μL。PLT 数量几乎是 WBC 数量的 10 倍。RBC 数量几乎是 WBC 的 3 倍[RBC/WBC 比值=4.67±3.58，PLT/WBC 比值=10.65±12.46]。经过 Histopaque 分离后，各层的活性依次为 WBC> RBC> PLT 进行 99mTc-HMPAO 标记。WBCs、RBCs 和 PLTs 的总活性/细胞数分别为 0.016±0.010、0.005±0.005 和 0.003±0.002（p>0.05）。