Kim Eun-Mi, Oh Phil-Sun, Boud Fatima, Jeong Hwan-Jeong, Lim Seok-Tae, Sohn Myung-Hee
Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Research Institute of Clinical Medicine of Jeonbuk National University and Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, Jeonbuk Republic of Korea.
Nucl Med Mol Imaging. 2020 Jun;54(3):147-155. doi: 10.1007/s13139-020-00645-8. Epub 2020 May 29.
The objective of this study was to describe to develop methods of rodent leukocyte isolation and radiolabeling for in vivo inflammation imaging.
Thigh muscle inflammation was induced by injection of collagenase. Blood was collected from the jugular vein and separated by Histopaque. The collected cells were incubated in a 37 °C CO incubator for 1~2 h. After incubation, Tc-HMPAO and F-FDG were used to treat leukocytes followed by incubation for 30 min. Tc-HMPAO and F-FDG labeled autologous leukocytes were injected into the tail veins of rats. The images were then acquired at various time points. Image-based lesion to normal muscle ratio was compared.
After Histopaque separation, the proportion of lymphocytes was higher than that of other cell types. After CO incubation, the collected leukocytes were viable, while room temperature exposed leukocytes without CO incubation were non-viable. Granulocytes, especially, were more quickly influenced by various conditions than the mononuclear cells. Labeling efficiencies of Tc-HMPAO and F-FDG were 4.00 ± 2.06 and 1.8%, respectively. Tc-HMPAO- and F-FDG-labeled leukocytes targeted well the inflamed lesion. Tc-HMPAO-labeled leukocytes, but not F-FDG-labeled leukocytes, were found in the abdomen activity.
Inflamed lesions of rats were well visualized using autologous radiolabeled leukocytes. This method might provide good information for understanding inflammatory diseases.
本研究的目的是描述开发用于体内炎症成像的啮齿动物白细胞分离和放射性标记方法。
通过注射胶原酶诱导大腿肌肉炎症。从颈静脉采集血液并用Histopaque分离。将收集的细胞在37°C二氧化碳培养箱中孵育1至2小时。孵育后，用锝-六甲基丙烯胺肟（Tc-HMPAO）和氟代脱氧葡萄糖（F-FDG）处理白细胞，然后再孵育30分钟。将Tc-HMPAO和F-FDG标记的自体白细胞注入大鼠尾静脉。然后在不同时间点采集图像。比较基于图像的病变与正常肌肉的比值。
经Histopaque分离后，淋巴细胞的比例高于其他细胞类型。经二氧化碳孵育后，收集的白细胞具有活力，而未经二氧化碳孵育且暴露于室温的白细胞则无活力。尤其是粒细胞比单核细胞更容易受到各种条件的快速影响。Tc-HMPAO和F-FDG的标记效率分别为4.00±2.06和1.8%。Tc-HMPAO和F-FDG标记的白细胞很好地靶向炎症病变。在腹部活动中发现了Tc-HMPAO标记的白细胞，但未发现F-FDG标记的白细胞。
使用自体放射性标记的白细胞可以很好地观察到大鼠的炎症病变。该方法可能为理解炎症性疾病提供良好的信息。
