一种用于在炎症细胞、斑马鱼和小鼠血液样本中检测和成像脂多糖诱导的HS的有效荧光传感器。
An effective fluorescent sensor for lipopolysaccharide-induced HS detection and imaging in inflammatory cells, zebrafish, and mouse blood samples.
作者信息
Zou Xinrong, Pang Meiling, He Jun, Kang Zhuxi, Wumaier Gulixiayida, Xue Shenglai, Yuan Lingli
机构信息
Department of Hematology, The Second Xiangya Hospital, Central South University Changsha Hunan 410011 PR China
Institute of Molecular Hematology, Central South University Changsha Hunan 410011 PR China.
出版信息
RSC Adv. 2025 Jul 21;15(32):25863-25871. doi: 10.1039/d5ra03905c.
In this work, a new fluorescent sensor (NAHCP) for the recognition of lipopolysaccharide-induced HS detection and imaging in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples was constructed. Based on the -aldehyde group (-CHO) auxiliary 2,4-dinitrophenyl ether (DNP) group sulfide cleavage, this sensor shows high sensitivity and selectivity towards HS, which can realize fluorescence "turn-on" detection. This sensor also features a very high response speed (∼2 min) and a large Stokes shift (113 nm) toward HS. Additionally, the excellent detection limit (LOD) for HS is as low as 25.6 nM. Impressively, this -CHO auxiliary DNP group sulfide cleavage ensemble is further successfully applied for HS detection in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples.
在这项工作中，构建了一种新型荧光传感器（NAHCP），用于在炎症性Raw264.7细胞、斑马鱼和小鼠血液样本中识别脂多糖诱导的HS检测及成像。基于醛基（-CHO）辅助的2,4-二硝基苯基醚（DNP）基团硫化物裂解，该传感器对HS表现出高灵敏度和选择性，可实现荧光“开启”检测。该传感器对HS还具有非常高的响应速度（约2分钟）和大斯托克斯位移（113 nm）。此外，HS的出色检测限（LOD）低至25.6 nM。令人印象深刻的是，这种-CHO辅助的DNP基团硫化物裂解体系进一步成功应用于炎症性Raw264.7细胞、斑马鱼和小鼠血液样本中的HS检测。
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