Sun Yong, Deng Zeyuan, Liu Ronghua, Zhang Hua, Zhu Honghui, Jiang Li, Tsao Rong
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China; Guelph Research and Development Centre, Agricultural and Agri-Food Canada, 93 Stone Road West, Guelph, ON N1G 5C9, Canada.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
Food Chem. 2020 Apr 27;325:126925. doi: 10.1016/j.foodchem.2020.126925.
A systemic approach was taken in profiling the hydrophilic and lipophilic antioxidants in lentil hulls using a combination of HPLC, LC-ESI-MS and GC techniques. A total of 37 phenolics were tentatively identified in the hydrophilic fractions, while four carotenoids and three tocopherols were found in the lipophilic fraction. Results showed that in addition to the high free extractable phenolics, phenolic compounds in conjugated and bound forms also exist in similar amounts. Information on conjugated and bound phenolics are particularly important as these forms of phenolics often go unnoticed by chromatographic profiling of extractables. All phenolic, carotenoid and tocopherol fractions contributed to antioxidant activities. Information about bioactives from lentil hulls, specifically conjugated and bound phenolics are reported here for the first time. The comprehensive profiling of these bioactives lays a good foundation for further assessment of the value-added uses of lentil hulls which are by-products of pulse processing.
采用HPLC、LC-ESI-MS和GC技术相结合的方法，对小扁豆壳中的亲水性和亲脂性抗氧化剂进行了系统分析。在亲水性部分初步鉴定出37种酚类物质，而在亲脂性部分发现了4种类胡萝卜素和3种生育酚。结果表明，除了大量可自由提取的酚类物质外，共轭态和结合态的酚类化合物含量也相近。共轭态和结合态酚类物质的信息尤为重要，因为这些酚类物质在可提取物的色谱分析中常常被忽视。所有酚类、类胡萝卜素和生育酚部分都具有抗氧化活性。本文首次报道了来自小扁豆壳的生物活性成分信息，特别是共轭态和结合态酚类物质。这些生物活性成分的全面分析为进一步评估作为豆类加工副产品的小扁豆壳的增值用途奠定了良好基础。
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