Zhao Jiarui, Wang Xinhe, Wang Yuchen, Lv Guangfu, Lin He, Lin Zhe
College of Pharmacy, Changchun University of Chinese Medicine, Changchun, China.
Front Nutr. 2023 Feb 1;10:1092071. doi: 10.3389/fnut.2023.1092071. eCollection 2023.
Hazel leaf, one of the by-products of hazelnut, which is widely used in traditional folk medicine around the world. In the present study, the profile of free, conjugated, and bound phenolic compounds from hazel leaf was detected and their antioxidant and anti-inflammatory activities were investigated. The potential health benefits of different phenolic compounds were also predicted. The results showed that the 35 phenolic substances of free, conjugated and bound forms were identified including phenolic acids, flavonoids and catechins. Most of the hazel leaf phenolics were presented in free form, followed by conjugated and bound form. All the fractions effectively inhibited the production of reactive oxygen species and malondialdehyde in TBHP-stimulated human umbilical vein endothelial cells by enhancing endogenous superoxide dismutase, and accordingly alleviated inflammatory cytokines (NO, IL-1β, TNF-α, and IL-6) in LPS-stimulated RAW264.7 cells, showing obvious antioxidant and anti-inflammatory capacity. Moreover, combined with network pharmacology, the potential therapeutic effects and functional pathways of hazel leaf phenolics were predicted, which provided value basis for exploring their treatment on diseases and developing health products in the future.
榛叶是榛子的副产品之一，在世界各地的传统民间医学中广泛应用。在本研究中，检测了榛叶中游离、结合和键合酚类化合物的概况，并研究了它们的抗氧化和抗炎活性。还预测了不同酚类化合物的潜在健康益处。结果表明，共鉴定出35种游离、结合和键合形式的酚类物质，包括酚酸、黄酮类化合物和儿茶素。大多数榛叶酚类物质以游离形式存在，其次是结合形式和键合形式。所有组分通过增强内源性超氧化物歧化酶，有效抑制了叔丁基过氧化氢刺激的人脐静脉内皮细胞中活性氧和丙二醛的产生，并相应减轻了脂多糖刺激的RAW264.7细胞中的炎性细胞因子（一氧化氮、白细胞介素-1β、肿瘤坏死因子-α和白细胞介素-6），显示出明显的抗氧化和抗炎能力。此外，结合网络药理学，预测了榛叶酚类物质的潜在治疗作用和功能途径，为今后探索其对疾病的治疗作用和开发健康产品提供了价值依据。
