Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, 832002, China.
Shanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest Agriculture and Forestry University, Yangling, Shaanxi, 712100, China.
Anal Chim Acta. 2020 Jun 15;1116:9-15. doi: 10.1016/j.aca.2020.04.030. Epub 2020 Apr 13.
The malondialdehyde (MDA)-specific detection probe (MDA-6) was successfully synthesised through the photoinduced electron transfer (PET) mechanism which possesses many biological applications. In vivo biological applicability of this probe was proved in different cell lines, zebrafish and mice. In these models, the MDA was produced by oxygen stress injury and the relationship between MDA and probe were evaluated in vitro as well as in vivo under different stress conditions. After comparing evaluated results with commercial MDA kit, MDA-6 was concluded with high specificity, low limit of detection (0.03 μM), and can achieve micro-detection of MDA with low cytotoxicity, demonstrating MDA-6 enables safe and effective detection.
通过光诱导电子转移（PET）机制成功合成了丙二醛（MDA）特异性检测探针（MDA-6），它具有许多生物应用。该探针在不同的细胞系、斑马鱼和小鼠中进行了体内生物适用性的证明。在这些模型中，氧应激损伤产生 MDA，评估 MDA 与探针之间的关系，包括在不同应激条件下的体外和体内。与商业 MDA 试剂盒的评估结果进行比较后，得出 MDA-6 具有高特异性、低检测限（0.03 μM），并且可以实现 MDA 的微检测，具有低细胞毒性，表明 MDA-6 能够进行安全有效的检测。
