Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering , University of Jinan , Shandong 250022 , P.R. China.
Anal Chem. 2019 Feb 5;91(3):1719-1723. doi: 10.1021/acs.analchem.8b05465. Epub 2019 Jan 17.
PDMS is biocompatible, economically viable, transparent, and facile to handle and thus is suitable for fluorescent microscopy and biological research. However, there has been no report about polysiloxane-based fluorescent probes applied in bioimaging. In this report, a two-photon polysiloxane-based reversible luminescent probe (P1) was fabricated for the first time. P1 is a powerful tool for detecting the ClO/GSH cycle in situ both in live cells and in zebrafish. This work demonstrates the potential of polysiloxane-based fluorescent probes for versatile in vivo or in vitro applications in the future.
PDMS 具有生物相容性、经济可行性、透明性和易于操作等特点，因此适用于荧光显微镜和生物学研究。然而，目前尚无关于基于聚硅氧烷的荧光探针在生物成像中的应用的报道。在本报告中，我们首次制备了一种基于聚硅氧烷的双光子可逆发光探针（P1）。P1 是一种强大的工具，可用于在活细胞和斑马鱼中实时检测 ClO/GSH 循环。这项工作展示了基于聚硅氧烷的荧光探针在未来用于各种体内或体外应用的潜力。
