College of Fisher and Life Science, Dalian Ocean University, Dalian, China.
Naturwissenschaften. 2024 Sep 9;111(5):46. doi: 10.1007/s00114-024-01932-6.
Megaloptera larvae are important bioindicator species and potential resource insects. To further cultivate their economic role, their living environment must be examined in more detail. In this study, we analyzed the physiological and biochemical effects of a sublethal dose of imidacloprid, a widely used neonicotinoid insecticide, on the larvae of Protohermes xanthodes. After treatment with imidacloprid, P. xanthodes larvae exhibited clear symptoms of poisoning, including the head curling up toward the ventral surface. Additionally, the activity of acetylcholinesterase was significantly inhibited following exposure. The activities of glutathione S-transferases initially continuously increased but showed a slight decrease after 8 days. Catalase activity initially increased and then decreased following imidacloprid treatment; superoxide dismutase activity fluctuated over time, and peroxidase activity continuously increased. The expression levels of HSP70s genes were evaluated using qRT-PCR. These results indicate that P. xanthodes larvae exhibit a toxic response to imidacloprid exposure, manifested as oxidative stress, as observed through behavioral and physiological indicators.
巨蝽幼虫是重要的生物指示物种和有潜力的资源昆虫。为了进一步挖掘它们的经济作用，必须更详细地研究它们的生活环境。在这项研究中，我们分析了一种广泛使用的新烟碱类杀虫剂——吡虫啉对黄缘巨蝽幼虫的亚致死剂量的生理生化效应。吡虫啉处理后，黄缘巨蝽幼虫表现出明显的中毒症状，包括头部向腹面卷曲。此外，暴露后乙酰胆碱酯酶的活性显著受到抑制。谷胱甘肽 S-转移酶的活性最初持续增加，但在 8 天后略有下降。过氧化氢酶活性在吡虫啉处理后先增加后减少；超氧化物歧化酶活性随时间波动，而过氧化物酶活性持续增加。使用 qRT-PCR 评估了 HSP70s 基因的表达水平。这些结果表明，黄缘巨蝽幼虫对吡虫啉暴露表现出毒性反应，表现为氧化应激，可通过行为和生理指标观察到。
