[特应性过敏性疾病患者血浆中激肽释放酶-激肽系统的激活机制]
[The mechanism of activation of kallikrein-kinin system in the plasma of patients with atopic allergic diseases].
作者信息
Dotsenko V L, Nenasheva N M, Neshkova E A, Morozova N A, Iarovaia G A
出版信息
Vopr Med Khim. 1988 Nov-Dec;34(6):89-94.
Data early obtained on activation of the kallikrein-kinin system in acute forms of pollinosis and urticaria were corroborated in vitro. These experiments exhibited enzymatic activation of Hageman factor and prekallikrein using "liberator", obtained after incubation of the leukocyte fraction enriched with basophils and specific allergen. I ml of the "liberator" containing 10(7) cells activated Hageman factor up to 12-20 mU per min (evaluated by BAEE-esterase activity of the kallikrein developed) as well as kallikrein activity was increased up to 80-130 mU within 1.5-2 hrs of incubation. The Hageman factor was distinctly inactivated after long-term incubation with the "liberator".
早期在急性花粉症和荨麻疹中获得的有关激肽释放酶-激肽系统激活的数据在体外得到了证实。这些实验显示，使用“释放剂”可实现哈格曼因子和前激肽释放酶的酶促激活，该“释放剂”是富含嗜碱性粒细胞的白细胞组分与特异性变应原孵育后获得的。1毫升含有10⁷个细胞的“释放剂”每分钟可将哈格曼因子激活至12 - 20毫单位（通过所产生的激肽释放酶的BAEE酯酶活性评估），并且在孵育1.5 - 2小时内激肽释放酶活性增加至80 - 130毫单位。与“释放剂”长期孵育后，哈格曼因子明显失活。
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