使用四种免疫层析测定法检测抗 SARS-CoV-2 抗体的动态分析。
Dynamic profile for the detection of anti-SARS-CoV-2 antibodies using four immunochromatographic assays.
机构信息
Department of Virology, Amiens University Medical Center, Amiens, France; AGIR Research Unit, EA4294, Jules Verne University of Picardie, Amiens, France.
Department of Gynecology-Obstetrics, University Hospital, Amiens.
出版信息
J Infect. 2020 Aug;81(2):e6-e10. doi: 10.1016/j.jinf.2020.04.033. Epub 2020 May 7.
In order to fight the SARS-CoV-2 pandemic infection, there is a growing need and demand for diagnostic tools that are complementary and different from the RT-PCR currently in use. Multiple serological tests are or will be very soon available but need to be evaluated and validated. We have thus tested 4 immunochromatographic tests for the detection of antibodies to SARS-CoV-2. In addition, we assessed the kinetics of antibody appearance using these assays in 22 patients after they were tested positive by RT-PCR. We observed great heterogeneity in antibody detection post-symptom onset. The median antibody detection time was between 8 and 10 days according to the manufacturers. All the tests showed a sensitivity of 60 to 80% on day 10 and 100% on day 15. In addition, a single cross-reaction was observed with other human coronavirus infections. Thus, immunochromatographic tests for the detection of anti-SARS-CoV-2 antibodies may have their place for the diagnostic panel of COVID-19.
为了对抗 SARS-CoV-2 大流行感染，人们对诊断工具的需求日益增长，这些工具与目前使用的 RT-PCR 不同，互为补充。目前有多种血清学检测方法已经或即将问世，但需要进行评估和验证。因此，我们测试了 4 种免疫层析检测法用于检测 SARS-CoV-2 抗体。此外，我们使用这些检测方法评估了 22 例 RT-PCR 阳性患者在出现症状后的抗体出现动力学。我们观察到在出现症状后抗体检测存在很大的异质性。根据制造商的说法，抗体检测的中位时间在 8 至 10 天之间。所有的检测在第 10 天的敏感性为 60%至 80%，在第 15 天的敏感性为 100%。此外，与其他人类冠状病毒感染也存在单个交叉反应。因此，用于检测抗 SARS-CoV-2 抗体的免疫层析检测法可能在 COVID-19 的诊断中占有一席之地。
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