Institute of Electronics, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Research & Development Center of Biomedical Photonics, Orel State University, Orel, Russia.
J Biophotonics. 2020 Aug;13(8):e202000082. doi: 10.1002/jbio.202000082. Epub 2020 May 28.
This work is dedicated to the diagnosis and grading of colon cancer by a combined use of Poincaré sphere and 2D Stokes vector polarimetry mapping approaches. The major challenge consists in exploring the applicability of polarized light for noninvasive screening of the histological abnormalities within the samples of biological tissues. Experimental studies were conducted in ex vivo colon sample, excised after surgical procedure for colon tumor removal of G2-adenocarcinoma lesion. Polarimetric measurements in linear and circular regime were carried via personally developed polarimetric, optical set-up, using supercontinuous fiber laser with irradiation fixed at 635 nm. We apply the Poincaré sphere and two-dimensional Stokes vector scanning approach for screening the corresponding tissue samples. A comparison between linear and circular polarization states is made both for quantitative and qualitative evaluations. It is shown that circular polarization has better diagnostic capabilities than linear polarization, with higher dynamic ranges of the polarimetric parameters and better values of the diagnostic quantities. In addition to the standard polarimetry parameters, utilized as essential diagnostic markers, we apply statistical analysis to obtain more detailed information in frame of the applied diagnostic approach.
这项工作致力于通过 Poincaré 球和二维 Stokes 向量极偏映射方法的组合来诊断和分级结肠癌。主要挑战在于探索偏振光在非侵入性筛查生物组织样本中组织学异常方面的适用性。实验研究是在离体结肠样本中进行的，这些样本是在结肠肿瘤切除 G2-腺癌病变的手术后切除的。通过个人开发的偏振光学装置，在 635nm 的固定照射下，使用超连续光纤激光器进行线性和圆偏振测量。我们应用 Poincaré 球和二维 Stokes 向量扫描方法来筛选相应的组织样本。对线性和圆偏振状态进行了定量和定性评估。结果表明，圆偏振比线偏振具有更好的诊断能力，具有更高的极偏参数动态范围和更好的诊断量值。除了作为基本诊断标记的标准偏振参数外，我们还应用统计分析来获得应用诊断方法的更详细信息。
