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来自[具体植物名称未完整给出]的多糖的体外纯化、表征及抗氧化活性

Purification, characterization and antioxidant activities in vitro of polysaccharides from L.

作者信息

Tang Zizhong, Zhou Caixia, Cai Yi, Tang Yujia, Sun Wenjun, Yao Huipeng, Zheng Tianrun, Chen Hui, Xiao Yirong, Shan Zhi, Bu Tongliang, Wang Xiaoli, Huang Lin, Gou Lin

机构信息

College of Life Sciences, Sichuan Agricultural University, Yaan, China.

Sichuan Agricultural University Hospital, Yaan, China.

出版信息

PeerJ. 2020 Apr 29;8:e9077. doi: 10.7717/peerj.9077. eCollection 2020.

DOI:10.7717/peerj.9077
PMID:32391207
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7195838/
Abstract

BACKGROUND

L. is an annual, erect or less commonly ascending herb that is a member of the family. Polysaccharides extracted from traditional Chinese medicines may be effective substances with antioxidant activity.

METHODS

In this study, we isolated crude polysaccharides from (AHP-M) using microwave-assisted extraction. Then, the AHP-M was purified by chromatography with DEAE-32 cellulose, and two fractions, AHP-M-1 and AHP-M-2, were obtained. The structural characteristics of AHP-M-1 and AHP-M-2 were investigated, and their antioxidant activities were analyzed in vitro.

RESULTS

We found that the monosaccharide composition of AHP-M-1 was different from that of AHP-M-2. The molecular weights of AHP-M-1 and AHP-M-2 were 77.625 kDa and 93.325 kDa, respectively. The results showed that the antioxidant activity of AHP-M-2 was better than that of AHP-M-1. For AHP-M-2, the DPPH radical scavenging rate at a concentration of 2 mg/mL was 78.87%, the hydroxyl radical scavenging rate was 39.34%, the superoxide anion radical scavenging rate was 80.2%, and the reduction ability of Fe was approximately 0.90. The total antioxidant capacity per milligram of AHP-M-2 was 6.42, which was higher than that of Vitamin C (Vc).

CONCLUSION

The in vitro test indicated that AHP-M-1 and AHP-M-2 have good antioxidant activity, demonstrating that L. polysaccharide has immense potential as a natural antioxidants.

摘要

背景

L. 是一种一年生直立草本植物，较少为斜升草本，属于该科植物。从中药中提取的多糖可能是具有抗氧化活性的有效物质。

方法

在本研究中，我们采用微波辅助提取法从（AHP - M）中分离出粗多糖。然后，通过DEAE - 32纤维素柱色谱法对AHP - M进行纯化，得到两个级分，即AHP - M - 1和AHP - M - 2。研究了AHP - M - 1和AHP - M - 2的结构特征，并对其体外抗氧化活性进行了分析。

结果

我们发现AHP - M - 1的单糖组成与AHP - M - 2不同。AHP - M - 1和AHP - M - 2的分子量分别为77.625 kDa和93.325 kDa。结果表明，AHP - M - 2的抗氧化活性优于AHP - M - 1。对于AHP - M - 2，浓度为2 mg/mL时的DPPH自由基清除率为78.87%，羟自由基清除率为39.34%，超氧阴离子自由基清除率为80.2%，铁还原能力约为0.90。每毫克AHP - M - 2的总抗氧化能力为6.42，高于维生素C（Vc）。

结论

体外试验表明，AHP - M - 1和AHP - M - 2具有良好的抗氧化活性，表明L. 多糖作为天然抗氧化剂具有巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/0206a58fd624/peerj-08-9077-g012.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/a72a77c7e8f5/peerj-08-9077-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/fc29e535ce5d/peerj-08-9077-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/dc75816de7d2/peerj-08-9077-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/dc3b4c12d749/peerj-08-9077-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/3726ab8cdda7/peerj-08-9077-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/d824326af292/peerj-08-9077-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/6485b46529b5/peerj-08-9077-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/0206a58fd624/peerj-08-9077-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/8ddf32985510/peerj-08-9077-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/35b963c978d6/peerj-08-9077-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/ff7f72af12b1/peerj-08-9077-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/96bc6bef1f8a/peerj-08-9077-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/a72a77c7e8f5/peerj-08-9077-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/fc29e535ce5d/peerj-08-9077-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/dc75816de7d2/peerj-08-9077-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/dc3b4c12d749/peerj-08-9077-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/3726ab8cdda7/peerj-08-9077-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/d824326af292/peerj-08-9077-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/6485b46529b5/peerj-08-9077-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2401/7195838/0206a58fd624/peerj-08-9077-g012.jpg

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