Abubakar Aminu Shehu, Huang Xiaoyu, Birhanie Ziggiju Mesenbet, Gao Gang, Feng Xinkang, Yu Chunming, Chen Ping, Chen Jikang, Chen Kunmei, Wang Xiaofei, Zhu Aiguo
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
Department of Agronomy, Bayero University Kano, Kano PMB 3011, Nigeria.
Plants (Basel). 2022 Jul 28;11(15):1964. doi: 10.3390/plants11151964.
a traditional medicinal plant used primarily as tea. It has a potential health benefit from its rich bioactive substances. This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the leaf. The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC). The result revealed the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides. Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW). It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively. Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract. The principal component analysis revealed a positive correlation between the constituents, bioactivities, and extracts. The overall result showed as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
一种主要用作茶的传统药用植物。因其富含生物活性物质而具有潜在的健康益处。本研究调查了该植物叶片不同极性溶剂（乙醇、乙酸乙酯、正己烷、甲醇和水）提取物的反应活性。使用傅里叶变换红外光谱仪（FT-IR）、气相色谱-质谱联用仪（GC-MS）、超高效液相色谱-质谱联用仪（UHPLC-MS）和高效液相色谱仪（HPLC）对提取物的植物化学成分进行了评估。结果显示存在具有重要药用价值的生物活性成分，包括酚类、黄酮类和多糖。甲醇提取物的黄酮类含量最高（20.11±0.85毫克槲皮素当量/克干重），酚类（9.25±0.03毫克没食子酸当量/克干重）和多糖（119.66±2.65毫克葡萄糖当量/克干重）含量次之。基于2,2-二苯基-1-苦基肼基（DPPH）自由基清除试验和铁还原抗氧化能力（FRAP），其抗氧化能力也最高，分别为60.30±0.52%和4.60±0.02微摩尔铁/克干重。乙醇提取物对革兰氏阴性菌和革兰氏阳性菌表现出最大的抗菌作用，对酪氨酸酶和乙酰胆碱酯酶的抑制活性最高，其次是甲醇提取物。主成分分析表明成分、生物活性和提取物之间存在正相关。总体结果表明该植物是抗菌和抗氧化生物活性化合物的丰富天然来源，可用于未来的制药和食品工业应用。
