Preeminent Medical Photonics Education & Research Center, Institute for NanoSuit Research, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Shizuoka, Japan.
Departments of Bio- and Material Photonics, Chitose Institute of Science and Technology, Chitose, Hokkaido, Japan.
PLoS One. 2020 May 11;15(5):e0232992. doi: 10.1371/journal.pone.0232992. eCollection 2020.
Although scanning electron microscopy (SEM) can generate high-resolution images of nanosized objects, it requires a high vacuum to do so, which precludes direct observations of living organisms and often produces unwanted structural changes. It has previously been reported that a simple surface modification gives rise to a nanoscale layer, termed the "NanoSuit", which can keep small animals alive under the high vacuum required for field-emission scanning electron microscopy (FE-SEM). We have previously applied this technique to plants, and successfully observed healthy petals in a fully hydrated state using SEM. The flower petals protected with the NanoSuit appeared intact, although we still lack a fundamental understanding of the images of other plants observed using FE-SEM. This report presents and evaluates a rich set of images, acquired using the NanoSuit, for a taxonomically diverse set of plant species. This dataset of images allows the surface features of various plants to be analyzed and thus provides a further complementary morphological profile. Image data can be accessed and viewed through Figshare (https://doi.org/10.6084/m9.figshare.c.4446026.v1).
尽管扫描电子显微镜 (SEM) 可以生成纳米级物体的高分辨率图像，但它需要高真空才能做到这一点，这就排除了对活生物体的直接观察，并且经常会产生不必要的结构变化。先前已经有报道称，简单的表面修饰会产生一个纳米级的层，称为“NanoSuit”，它可以使小动物在场发射扫描电子显微镜 (FE-SEM) 所需的高真空下存活。我们之前将这项技术应用于植物，并成功地使用 SEM 观察到完全水合状态下的健康花瓣。用 NanoSuit 保护的花瓣看起来完好无损，尽管我们仍然缺乏对使用 FE-SEM 观察到的其他植物图像的基本了解。本报告介绍并评估了一组使用 NanoSuit 获取的、具有丰富多样性的植物物种的图像。该图像数据集允许分析各种植物的表面特征，从而提供进一步的互补形态学特征。图像数据可通过 Figshare（https://doi.org/10.6084/m9.figshare.c.4446026.v1）访问和查看。
