Laboratory of Bio-Design, Department of Agricultural Innovation for Sustainable Society, Tokyo University of Agriculture, 1737 Funako, Atsugi, Kanagawa, 243-0034, Japan.
NanoSuit Inc., 1-20-1 Handayama, Higashi-ku, Hamamatsu, 431-3192, Japan.
Sci Data. 2024 Sep 27;11(1):1053. doi: 10.1038/s41597-024-03900-1.
A simple surface modification, called NanoSuit, by electron beam or by plasma irradiation can form a nanoscale layer, allowing to keep small animals alive and hydrous under the high vacuum required for field-emission scanning electron microscopy (FE-SEM). We previously applied NanoSuit to aquatic insects, Dixa longistyla larvae (Diptera: Dixidae), which always lie on their ventral surface just under the water surface. We found that the crown-like structures on the ventral side of the hind segments enable the larvae to reside in such ecological niche. Moreover, fine structures in the crown protected with NanoSuit appeared intact, unlike those subjected to conventional sample fixation. However, a fundamental understanding of these structures (living and/or not treated with conventional fixation) interacting directly with water should be established using FE-SEM. This data descriptor introduces a rich dataset of images acquired using NanoSuit for various aquatic insects. The image data can be accessed and viewed through Figshare.
一种简单的表面改性方法，称为 NanoSuit，通过电子束或等离子体辐照可以形成纳米级层，使小动物在用于场发射扫描电子显微镜（FE-SEM）的高真空下保持存活和含水状态。我们之前将 NanoSuit 应用于水生昆虫，长角蝇幼虫（双翅目：蝇科），它们总是躺在水面下的腹面。我们发现，后段腹侧的冠状结构使幼虫能够栖息在这样的生态位中。此外，用 NanoSuit 保护的冠状精细结构保持完整，与未经常规样品固定处理的结构不同。然而，应该使用 FE-SEM 直接建立对这些结构（活体和/或未经常规固定处理）与水相互作用的基本理解。本数据描述符介绍了使用 NanoSuit 为各种水生昆虫获取的丰富图像数据集。可以通过 Figshare 访问和查看图像数据。
