Turbat Adiyadolgor, Rakk Dávid, Vigneshwari Aruna, Kocsubé Sándor, Thu Huynh, Szepesi Ágnes, Bakacsy László, D Škrbić Biljana, Jigjiddorj Enkh-Amgalan, Vágvölgyi Csaba, Szekeres András
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary.
Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary.
Microorganisms. 2020 May 7;8(5):683. doi: 10.3390/microorganisms8050683.
Endophytic fungi in symbiotic association with their host plant are well known to improve plant growth and reduce the adverse effects of both biotic and abiotic stresses. Therefore, fungal endophytes are beginning to receive increased attention in an effort to find growth-promoting strains that could be applied to enhance crop yield and quality. In our study, the plant growth-promoting activities of endophytic fungi isolated from various parts of (a medicinally important plant in Mongolia and China) have been revealed and investigated. Fungal isolates were identified using molecular taxonomical methods, while their plant growth-promoting abilities were evaluated in plate assays. Altogether, 15 strains were isolated, representing the genera , and . Five of the isolates possessed phosphate solubilization activities and twelve secreted siderophores, while all of them were able to produce indoleacetic acid (IAA) in the presence or absence of tryptophan. The endogenous and exogenous accumulation of IAA were also monitored in liquid cultures using the HPLC-MS/MS technique to refine the plate assay results. Furthermore, for the highest IAA producer fungi, the effects of their extracts were also examined in plant bioassays. In these tests, the primary root lengths of the model were increased in several cases, while the biomasses were significantly lower than the control IAA treatment. Significant alterations have also been detected in the photosynthetic pigment (chlorophyll-a, -b and carotenoids) content due to the fungal extract treatments, but these changes did not show any specific trends.
与宿主植物共生的内生真菌能够促进植物生长，并减轻生物和非生物胁迫的不利影响，这是众所周知的。因此，为了寻找可用于提高作物产量和品质的促生长菌株，真菌内生菌开始受到越来越多的关注。在我们的研究中，已经揭示并研究了从（一种在中国和蒙古具有重要药用价值的植物）不同部位分离出的内生真菌的植物促生长活性。使用分子分类学方法对真菌分离株进行鉴定，同时在平板试验中评估它们的植物促生长能力。总共分离出15个菌株，分别代表、和属。其中5个分离株具有溶解磷酸盐的活性，12个分泌铁载体，并且所有分离株在有无色氨酸的情况下都能够产生吲哚乙酸（IAA）。还使用HPLC-MS/MS技术在液体培养物中监测IAA的内源和外源积累，以完善平板试验结果。此外，对于IAA产量最高的真菌，还在植物生物测定中检测了它们提取物的效果。在这些试验中，在几种情况下模式植物的主根长度增加了，而生物量显著低于对照IAA处理。由于真菌提取物处理，光合色素（叶绿素a、b和类胡萝卜素）含量也检测到显著变化，但这些变化没有显示出任何特定趋势。
