Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt.
Biology Department, College of Science, Taibah University, Yanbu 41911, Saudi Arabia.
Biomolecules. 2021 Jan 22;11(2):140. doi: 10.3390/biom11020140.
Endophytic fungi are widely present in internal plant tissues and provide different benefits to their host. Medicinal plants have unexplored diversity of functional fungal association; therefore, this study aimed to isolate endophytic fungi associated with leaves of medicinal plants and evaluate their plant growth-promoting properties. Fifteen isolated fungal endophytes belonging to Ascomycota, with three different genera, and , were obtained from healthy leaves of . These fungal endophytes have varied antimicrobial activity against human pathogenic microbes and produce ammonia and indole acetic acid (IAA), in addition to their enzymatic activity. The results showed that EP-5 had a maximum IAA productivity of 192.1 ± 4.04 µg mL in the presence of 5 µg mL tryptophan. The fungal isolates of EP-2 EP-3, and EP-14 exhibited variable efficiency for solubilizing phosphate salts. Five representative fungal endophytes of EP-2, EP-5, EP-11, EP-13, and EP-14 and their consortium were selected and applied as bioinoculant to maize plants. The results showed that EP-5 increased root lengths from 15.8 ± 0.8 to 22.1 ± 0.6. Moreover, the vegetative growth features of inoculated maize plants improved more than the uninoculated ones.
内生真菌广泛存在于植物内部组织中，为其宿主提供各种益处。药用植物具有未被探索的功能真菌共生多样性；因此，本研究旨在从药用植物的叶片中分离与内生真菌相关的内生真菌，并评估其促进植物生长的特性。从 的健康叶片中获得了 15 株属于子囊菌门的内生真菌，属于三个不同的属， 和 。这些内生真菌对人类致病微生物具有不同的抗菌活性，并产生氨和吲哚乙酸（IAA），此外还具有酶活性。结果表明，在 5 µg mL 色氨酸存在的情况下，EP-5 的 IAA 产量最高，为 192.1 ± 4.04 µg mL。EP-2、EP-3 和 EP-14 的真菌分离株对磷酸盐盐的溶解效率不同。选择了 EP-2、EP-5、EP-11、EP-13 和 EP-14 这五个有代表性的内生真菌及其混合物作为生物接种剂应用于玉米植株。结果表明，EP-5 将根长从 15.8 ± 0.8 增加到 22.1 ± 0.6。此外，接种玉米植株的营养生长特征比未接种的要好。
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