Department of Agriculture, University of Patras, Theodoropoulou Str, 27200, Amaliada, Greece.
Laboratory of Genetics, Department of Biology, University of Patras, 26504, Patras, Greece.
Biochem Genet. 2020 Oct;58(5):725-737. doi: 10.1007/s10528-020-09970-1. Epub 2020 May 11.
Origanum L. (Lamiaceae) is an important genus of medicinal and aromatic plants used since ancient times as culinary herbs and remedies in traditional medicine. Although it is a relatively small genus, intra-generic species delineation, as well as its inter-generic relationships within tribe Mentheae, are still poorly understood. High resolution melting (HRM) analysis, coupled with microsatellite markers (SSRs), could facilitate the molecular identification and characterization of certain genotypes more efficiently and relatively faster when compared to other analytical methods. In this study, 38 Origanum samples corresponding to six Origanum taxa (O. dictamnus, O. majorana, O. onites, O. scabrum, O. sipyleum, and O. vulgare subsp. hirtum) were analyzed, using six microsatellite loci. Our goal was to molecularly identify and discriminate among the selected samples and to evaluate the ability of the HRM technique as an analytical tool for the discrimination of Origanum species from Greece. The temperature-shifted melting curves produced by the HRM analysis, resulted in 98 unique HRM profiles, which enabled the discrimination of the Origanum genotypes studied. According to the similarity dendrogram based on the HRM profiles, six unique clusters were formed, each one corresponding to a single taxon. In conclusion, HRM genotyping provided a fast, cost-effective method, well suited for the molecular characterization and identification of Origanum taxa and for the authentication of the original genetic material.
牛至属（唇形科）是一种重要的药用和芳香植物属，自古以来就被用作烹饪草药和传统医学中的药物。尽管它是一个相对较小的属，但种内物种划分以及在薄荷族内的种间关系仍然知之甚少。高分辨率熔解（HRM）分析，结合微卫星标记（SSR），与其他分析方法相比，可以更有效地对某些基因型进行分子鉴定和特征描述，并且速度相对较快。在这项研究中，使用六个微卫星位点分析了 38 个对应于六个牛至种（O. dictamnus、O. majorana、O. onites、O. scabrum、O. sipyleum 和 O. vulgare subsp. hirtum）的牛至样本。我们的目标是对选定的样本进行分子鉴定和区分，并评估 HRM 技术作为区分来自希腊的牛至物种的分析工具的能力。HRM 分析产生的温度偏移熔解曲线产生了 98 个独特的 HRM 图谱，这些图谱能够区分研究的牛至基因型。根据基于 HRM 图谱的相似性聚类树，形成了六个独特的聚类，每个聚类对应于一个单一的分类群。总之，HRM 基因分型提供了一种快速、具有成本效益的方法，非常适合牛至种的分子特征描述和鉴定，以及原始遗传物质的认证。
