Laboratory of Herbal Pesticides, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, U.P., 221005, India.
Centre For Plant Molecular Biology and Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, T.N., 641003, India.
Environ Sci Pollut Res Int. 2020 Aug;27(22):27635-27650. doi: 10.1007/s11356-020-08872-4. Epub 2020 May 11.
The study reports the preservative efficacy of Bunium persicum (Boiss) essential oil (BPEO) against fungal and aflatoxin B (AFB) contamination of stored masticatories and boosting of its efficacy through encapsulation into chitosan. BPEO was chemically characterized through GC-MS analysis, which revealed γ-terpinene as the major compound. The BPEO at 1.2 μL/mL concentration completely inhibited the growth of toxigenic strain of Aspergillus flavus (AF-LHP-PE-4) along with 15 common food borne moulds and AFB secretion. The BPEO exerts its antifungal action on plasma membrane, as confirmed through ergosterol inhibition, alteration of membrane fluidity and enhancement of cellular ions and 260 and 280 nm absorbing material leakage. The antiaflatoxigenic mechanism of action of BPEO was confirmed through methylglyoxal reduction. Further, BPEO showed strong antioxidant activity (IC = 7.36 μL/mL) as measured by DPPH assay. During in situ investigation, BPEO completely inhibited AFB production in model food (Phyllanthus emblica) system without altering the sensory properties and also exhibited high LD value (14,584.54 μL/kg) on mice. In addition, BPEO was encapsulated into chitosan, characterized and tested for their potential to inhibit growth and AFB production. The mean particle size, PDI and zeta potential of formed BPEO-loaded chitosan nanoparticle (CS-Np-BPEO) were performed to confirm successful encapsulation. The result revealed nanoencapsulated BPEO showed enhanced activity and completely inhibited the growth and AFB production by AF-LHP-PE-4 at 0.8 μL/mL. Based on findings, it could be concluded that the BPEO and its encapsulated formulation can be recommended as a potential plant-based preservative against fungal and aflatoxin contamination of stored masticatories.
该研究报告了 Bunium persicum (Boiss) 精油（BPEO）对储存的咀嚼物的真菌和黄曲霉毒素 B（AFB）污染的防腐效果，并通过将其封装到壳聚糖中来提高其功效。通过 GC-MS 分析对 BPEO 进行了化学表征，结果表明 γ-萜品烯是主要化合物。BPEO 在 1.2 μL/mL 浓度下完全抑制了产毒菌株黄曲霉（AF-LHP-PE-4）以及 15 种常见食源真菌和 AFB 分泌的生长。BPEO 通过抑制麦角固醇、改变膜流动性以及增强细胞离子和 260nm 和 280nm 吸收物质的渗漏来发挥其抗真菌作用。BPEO 的抗黄曲霉作用机制通过甲基乙二醛还原得到证实。此外，BPEO 通过 DPPH 测定显示出很强的抗氧化活性（IC=7.36 μL/mL）。在原位研究中，BPEO 完全抑制了模型食品（Phyllanthus emblica）系统中的 AFB 产生，而不会改变感官特性，并且在小鼠身上还表现出高 LD 值（14,584.54 μL/kg）。此外，将 BPEO 封装到壳聚糖中，对其抑制生长和 AFB 产生的潜力进行了表征和测试。形成的 BPEO 负载壳聚糖纳米颗粒（CS-Np-BPEO）的平均粒径、PDI 和zeta 电位用于确认成功封装。结果表明，纳米封装的 BPEO 在 0.8 μL/mL 时显示出增强的活性，并完全抑制了 AF-LHP-PE-4 的生长和 AFB 产生。根据研究结果可以得出结论，BPEO 及其包封制剂可以作为一种潜在的植物性防腐剂，用于防止储存的咀嚼物受到真菌和黄曲霉毒素的污染。
