Department of Biochemistry and Molecular Biology, Pondicherry University, Pondicherry, India.
Lett Appl Microbiol. 2020 Sep;71(3):272-279. doi: 10.1111/lam.13313. Epub 2020 Jun 15.
Impairment of antioxidant enzymes activities has been well reported in several human diseases. Effective anti-ageing strategies involving antioxidant supplementation and/or caloric restriction (CR) are receiving a great attention to mitigate free radical-mediated oxidative damage in several disease conditions to improve active longevity. Therefore, in this work, we have evaluated the protective effect of quercetin under non restriction (NR) and CR conditions on the sensitivity of Saccharomyces cerevisiae mutant strains (sod1∆, sod2∆, cta1∆, ctt1∆, tsa1∆ and glr1∆) deficient in antioxidant defence systems (superoxide dismutase, catalase, thioredoxin peroxidase and glutathione reductase) against H O -induced oxidative stress. Our results demonstrate that quercetin in combination with CR has strongly reduced the H O -mediated stress in the yeast mutant cells compared to NR conditions. Furthermore, we show that quercetin in combination with CR enhanced the percentage viability of yeast cells during chronological ageing. Our research findings suggest that antioxidant supplementation in combination with CR might have potent beneficial effects than individual therapies against free radical-mediated oxidative stress. SIGNIFICANCE AND IMPACT OF THE STUDY: Oxidative stress results from an imbalance between free radicals and antioxidant defense systems in our body. Supplementation with exogenous antioxidants is necessary to neutralize the free radical mediated damage. Polyphenols are a group of naturally occurring plant compounds with strong free radical-scavenging activity and exhibits potent anti-aging property by mitigating oxidative stress. On the other hand, caloric restriction (CR) has been reported to be a popular leading anti-aging approach to ameliorate age-associate macromolecular damages in various chronic human diseases. Evaluation of protective effects of antioxidant supplementation in combination with CR against free radical mediated oxidative stress is pivotal for the development of novel anti-aging strategies to improve active longevity.
抗氧化酶活性的损伤在许多人类疾病中已有报道。涉及抗氧化剂补充和/或热量限制（CR）的有效抗衰老策略受到极大关注，以减轻几种疾病条件下自由基介导的氧化损伤，从而提高活跃寿命。因此，在这项工作中，我们评估了在非限制（NR）和 CR 条件下槲皮素对酵母突变株（sod1∆、sod2∆、cta1∆、ctt1∆、tsa1∆和 glr1∆）抗氧化防御系统（超氧化物歧化酶、过氧化氢酶、硫氧还蛋白过氧化物酶和谷胱甘肽还原酶）缺陷的敏感性的保护作用，这些突变株对 H O 诱导的氧化应激敏感。我们的结果表明，与 NR 条件相比，槲皮素与 CR 联合使用大大降低了酵母突变细胞中 H O 介导的应激。此外，我们表明，槲皮素与 CR 联合使用增强了酵母细胞在生物钟老化过程中的存活率。我们的研究结果表明，抗氧化剂补充与 CR 联合使用可能比单独针对自由基介导的氧化应激的治疗具有更强的有益效果。
氧化应激是由于我们体内自由基和抗氧化防御系统之间的不平衡引起的。补充外源性抗氧化剂对于中和自由基介导的损伤是必要的。多酚是一组天然存在的植物化合物，具有很强的自由基清除活性，并通过减轻氧化应激表现出有效的抗衰老特性。另一方面，热量限制（CR）已被报道为一种流行的抗衰老方法，以改善各种慢性人类疾病中与年龄相关的大分子损伤。评估抗氧化剂补充与 CR 联合使用对自由基介导的氧化应激的保护作用对于开发新的抗衰老策略以提高活跃寿命至关重要。
